Effects of administration of transforming growth factor (TGF)-beta1 and anti-TGF-beta1 antibody on the mechanical properties of the stress-shielded patellar tendon.
Effects of administration of transforming growth factor (TGF)-beta1 and anti-TGF-beta1 antibody on the mechanical properties of the stress-shielded patellar tendon.
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DOI：
10.1016/j.jbiomech.2005.09.001
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发表时间：
2006
影响因子：
2.4
通讯作者：
Taro Katsura;H. Tohyama;Eiji Kondo;N. Kitamura;K. Yasuda
中科院分区：
文献类型：
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作者：
Taro Katsura;H. Tohyama;Eiji Kondo;N. Kitamura;K. Yasuda
先前的研究表明，在应力屏蔽的髌腱中，肌腱的力学性能显着降低，并且TGF-β在肌腱成纤维细胞中过度表达。因此，在本研究中，我们使用 40 只兔子测试了两个支持性假设：其一是应用 TGF-β1 可能会显着增加应力屏蔽髌腱的拉伸强度和切线模量。另一个问题是，施用抗 TGF-β1 抗体可能会显着降低应力屏蔽髌腱的机械性能。结果显示，与假治疗相比，应用 4 ng TGF-β1 在 3 周时显着增加了应力屏蔽髌腱的切线模量 (p=0.019)。关于拉伸强度，施用 4 ng TGF-β1 提高了平均值，但未达到统计学显着性。与假治疗相比，50 μg 抗 TGF-β1 抗体的应用显着降低了 3 周时应力屏蔽髌腱的切线模量和拉伸强度（p=0.0068 和 p=0.0355）。由于本研究中使用的应力屏蔽治疗显着降低了髌腱的切线模量和拉伸强度，本研究表明，TGF-β1的施用微弱但显着地抑制了应力屏蔽的髌腱机械性能的降低，并且TGF-β1及其抗体的失活显着增强了应力屏蔽的髌腱中发生的机械性能的降低。这些结果表明TGF-β1在应力屏蔽髌腱的重塑中发挥重要作用。
Previous studies have shown that, in the stress-shielded patellar tendon, the mechanical properties of the tendon were dramatically reduced and TGF-beta was over-expressed in tendon fibroblasts. In the present study, therefore, we tested two supportive hypotheses using 40 rabbits: One was that an application of TGF-beta1 might significantly increase the tensile strength and the tangent modulus of the stress-shielded patellar tendon. The other one was that an administration of anti-TGF-beta1 antibody might significantly reduce the mechanical properties of the stress-shielded patellar tendon. In the results, an application of 4-ng TGF-beta1 significantly increased the tangent modulus of the stress-shielded patellar tendon at 3 weeks (p=0.019), compared with the sham treatment. Concerning the tensile strength, the 4-ng TGF-beta1 application increased the average value, but a statistical significance was not reached. An application of 50-μg anti-TGF-beta1 antibody significantly reduced the tangent modulus and the tensile strength of the stress-shielded patellar tendon at 3 weeks (p=0.0068 and p=0.0355), compared with the sham treatment. Because the stress-shielding treatment used in this study dramatically reduces the tangent modulus and the tensile strength of the patellar tendon, the present study suggested that an administration of TGF-beta1 weakly but significantly inhibited the reduction of the mechanical properties of the stress-shielded patellar tendon, and that inactivation of TGF-beta1 with its antibody significantly enhanced the reduction of the mechanical properties that occurs in the stress-shielded patellar tendon. These results suggested that TGF-beta1 plays an important role in remodeling of the stress-shielded patellar tendon.