Ultrasound-induced DNA damage and signal transductions indicated by gammaH2AX
Ultrasound-induced DNA damage and signal transductions indicated by gammaH2AX
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gammaH2AX 表明超声诱导的 DNA 损伤和信号转导
DOI：
10.1063/1.3607926
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
T. Kondo
中科院分区：
文献类型：
--
作者：
Yukihiro Furusawa;Y. Fujiwara;Qing‐Li Zhao;M. Hassan;R. Ogawa;Y. Tabuchi;I. Takasaki;A. Takahashi;T. Ohnishi;T. Kondo
超声可通过不同的方式诱导癌细胞死亡，其中包括细胞凋亡。在这里，我们报告了低强度脉冲超声(LIPUS)诱导基因组DNA损伤和随后的DNA损伤反应的可能性。以电离辐射诱导的DNA双链断裂(DSB)为阳性对照，我们观察了LIPUS照射后人白血病细胞DNA双链断裂(DSB)的诱导(中性彗尾)和随后形成的γ-H_2AX阳性灶(通过免疫荧光检测)。LIPUS诱导的DNA损伤很可能是由于空化的机械效应，而不是声化学效应，因为我们观察到，抑制惯性空化消除了γ-H_2AX焦点的形成，而清除自由基形成(如羟基自由基)对其没有保护作用。用能磷酸化H_2AX Ser139的ATM或DNA-PKcs的特异性激酶抑制剂处理后，发现US诱导的H_2AX更有效地被抑制。
Ultrasound (US) has been shown to induce cancer cell death via different forms including apoptosis. Here, we report the potential of low‐intensity pulsed US (LIPUS) to induce genomic DNA damage and subsequent DNA damage response. Using the ionizing radiation‐induced DNA double‐strand breaks (DSBs) as the positive control, we were able to observe the induction of DSBs (as neutral comet tails) and the subsequent formation of gammaH2AX‐positive foci (by immunofluorescence detection) in human leukemia cells following exposure to LIPUS. The LIPUS‐induced DNA damage arose most likely from the mechanical, but not sonochemical, effect of cavitation, based on our observation that the suppression of inertial cavitation abrogated the gammH2AX foci formation, whereas scavenging of free radical formation (e.g., hydroxyl radical) had no protective effect on it. Treatment with the specific kinase inhibitor of ATM or DNA‐PKcs, which can phosphorylate H2AX Ser139, revealed that US‐induced gammaH2AX was inhibited more effe...
影响因子：
9.7
作者：
Ando, Hidetaka;Feril, Loreto B., Jr.;Misaki, Takuro
通讯作者：
Misaki, Takuro