Overexpression of the receptor for hyaluronan-mediated motility, correlates with expression of microtubule-associated protein in human oral squamous cell carcinomas
Overexpression of the receptor for hyaluronan-mediated motility, correlates with expression of microtubule-associated protein in human oral squamous cell carcinomas
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DOI：
10.3892/ijo_00000286
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发表时间：
2009-06-01
影响因子：
5.2
通讯作者：
Kamata, Nobuyuki
中科院分区：
文献类型：
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作者：
Shigeishi, Hideo;Fujimoto, Shinichi;Kamata, Nobuyuki
透明质酸介导的运动受体（RHAMM）以前被表征为透明质酸的细胞表面受体和微管相关的细胞内透明质酸结合蛋白。采用实时荧光定量RT-PCR方法检测了43例口腔鳞癌和7例正常牙龈组织中RHAMM mRNA的表达。RHAMM mRNA在口腔鳞癌中的表达水平显著高于正常牙龈组织（P=0.0047）。43例口腔SCC中有40例显示RHAMM剪接变异体（48 bp缺失）的表达。我们通过化学方法证实了RHAMM在口腔鳞状细胞癌中的蛋白表达，RHAMM mRNA水平较高。RHAMM高表达的口腔鳞癌患者的生存率低于低表达患者。然而，其不具有统计学显著性。已有报道RHAMM与纺锤体组装因子如微管相关蛋白（TPX 2）相互作用。为了研究微管相关蛋白在口腔SCC中的表达，还通过实时RT-PCR检测TPX 2的mRNA表达。TPX 2 mRNA在口腔鳞癌中的表达水平显著高于正常牙龈组织（P=0.046）。TPX 2和RHAMM的mRNA表达水平之间存在显著相关性（P=0.011）。结果表明，TPX 2和RHAMM在口腔SCC中的mRNA表达水平之间存在强相关性。提示RHAMM和TPX 2基因表达上调与口腔鳞癌的恶性程度有关，可能与口腔鳞癌细胞增殖异常或增殖增加有关。
Receptor for hyaluronan-mediated motility (RHAMM) has previously been characterized as a cell surface receptor for hyaluronan and a microtubule-associated intracellular hyaluronan binding protein. We examined the expression of RHAMM mRNA in 43 oral squamous cell carcinomas (SCCs) and 7 normal gingivae by real-time RT-PCR. The expression level of RHAMM mRNA was significantly higher in oral SCCs than normal gingivae (P=0.0047). Forty out of 43 oral SCCs showed expression of RHAMM splice variant (48 bp deletion). We immunohistochemically confirmed the protein expression of RHAMM in oral SCCs with higher levels of RHAMM mRNA. Patients with oral SCC who had high RHAMM expression had shorter survival rates than patients with low expression. However, it was not statistically significant. It has been reported that RHAMM interacts with spindle assembly factors such as microtubule-associated protein (TPX2). To investigate the expression of microtubule-associated protein in oral SCCs, mRNA expression of TPX2 was also examined by real-time RT-PCR. The expression level of TPX2 mRNA was significantly higher in oral SCCs than normal gingivae (P=0.046). Furthermore, a significant correlation between the mRNA expression levels of TPX2 and RHAMM was recognized (P=0.011). The results indicate that there is a strong correlation between the mRNA expression levels of TPX2 and RHAMM in oral SCCs. Our observations suggest that the up-regulations of human RHAMM and TPX2 gene correlate with the malignant condition and might be linked to the increased or abnormal cell proliferation in human oral SCCs.