Inducible gene deletion in astroglia and radial glia -: A valuable tool for functional and lineage analysis
Inducible gene deletion in astroglia and radial glia -: A valuable tool for functional and lineage analysis
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DOI：
10.1002/glia.20350
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发表时间：
2006-07-01
期刊：
影响因子：
6.2
通讯作者：
Goetz, Magdalena
中科院分区：
文献类型：
--
作者：
Mori, Tetsuji;Tanaka, Kohichi;Goetz, Magdalena
星形胶质细胞被认为在成人大脑中发挥着多种关键作用，如参与突触传递，参与脑损伤后的伤口愈合，以及成人神经发生。然而，由于缺乏星形胶质细胞特异性的基因靶向，在体内阐明这些功能一直是困难的。在这里，我们发现，在星形胶质细胞特异性谷氨酸转运体(GLAST)基因座上表达的Cre的诱导型形式(CreERT2)允许在成年星形胶质细胞和早期发育阶段的放射状胶质细胞中精确定时的基因缺失。此外，出生后和成人的神经发生可以在不同的阶段高效地靶向，因为它起源于星形胶质细胞。综上所述，这一小鼠品系将允许解剖调控星形胶质细胞作为前体、支持细胞、修复细胞和参与神经元信息处理的细胞的不同功能的分子通路。(C)2006年Wiley-Liss，Inc.
Astrocytes are thought to play a variety of key roles in the adult brain, such as their participation in synaptic transmission, in wound healing upon brain injury, and adult neurogenesis. However, to elucidate these functions in vivo has been difficult because of the lack of astrocyte-specific gene targeting. Here we show that the inducible form of Cre (CreERT2) expressed in the locus of the astrocyte-specific glutamate transporter (GLAST) allows precisely timed gene deletion in adult astrocytes as well as radial glial cells at earlier developmental stages. Moreover, postnatal and adult neurogenesis can be targeted at different stages wit high efficiency as it originates from astroglial cells. Taken together, this mouse line will allow dissecting the molecular pathways regulating the diverse functions of astrocytes as precursors, support cells, repair cells, and cells involved in neuronal information processing. (c) 2006 Wiley-Liss, Inc.