Rooting Induction of Soft Wood and Almond Micro-cutting Using Agrobacterium Rhizogenes
Rooting Induction of Soft Wood and Almond Micro-cutting Using Agrobacterium Rhizogenes
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发表时间：
2010
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通讯作者：
M. S. A. Rayya;N. Kassem;E. A. Ali
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作者：
M. S. A. Rayya;N. Kassem;E. A. Ali
目前的工作是在1999年至2003年期间在农业发展系统（ADS）的实验室进行的。开罗,埃及。研究了在大田和离体条件下，根生农杆菌诱导苦杏仁生根的效果。从这项工作的结果中得出以下结论。在露天条件下，感染根生杆菌的苦芒针叶扦插完全不能生根。结果表明，WP培养基生根率最高，根数/生根梢数最多，B、N、MS培养基次之，而B、N、WP培养基的根长最长。在生根培养基中添加活性炭可使生根率急剧下降。NAA对苦参生根率最高（40.74），根/根尖数最多，根/根尖长度最小，IBA次之，IAA则相反。用发根农杆菌w.t t，菌株1855在微插条根部侵染，对两种扁桃砧木的离体生根效果满意（95.00 ~ 99.00%）。
The present work was carried out during the period between 1999 t ill 2003 i n t he laboratories of Agriculture Development System (ADS). Cairo, Egypt. This study aimed to investigate rooting induction of bitter almond under open field and in vitro using Agrobacterium rhizogenes. The following conclusions were derived from the res ults of this work. Bitter al mond softwood cuttings infected with A grobacterium rhizogenes failed completely to root under open field conditions.WP medium proved to be suitable rooting medium for rooting bitter alm ond shoots followed by B & N and MS respectively as it gave the highest rooting percentage, number of roots / rooted shoot while B & N and W P medi um g ave the longest root length. Adding activated charcoal to the rooting medium caused a sharp decrease in the rooting percentage. NAA wa s the most effective auxin in inducing the best rooting percentage (40.74), greatest number of roots / rooted shoot and the lo ngest root of bitter al mond shoots followed by IBA, while IAA took t he other way round. Satisfactory in v itro root ing (95.00- 99.00 %) was obtained in bi tter almond r ootstock through the infection of Agrobacterium rhizogenes w. t., strain 1855 at the base of micro - cuttings.