Interaction with the Na, K-ATPase and tissue distribution of FXYD5 (related to ion channel)
Interaction with the Na, K-ATPase and tissue distribution of FXYD5 (related to ion channel)
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DOI：
10.1074/jbc.m506397200
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发表时间：
2005-11-11
影响因子：
4.8
通讯作者：
Garty, H
中科院分区：
文献类型：
--
作者：
Lubarski, I;Pihakaski-Maunsbach, K;Garty, H
FXYD5 ( related to ion channel, dysadherin) is a member of the FXYD family of single span type I membrane proteins. Five members of this group have been shown to interact with the Na, K-ATPase and to modulate its properties. However, FXYD5 is structurally different from other family members and has been suggested to play a role in regulating E-cadherin and promoting metastasis ( Ino, Y., Gotoh, M., Sakamoto, M., Tsukagoshi, K., and Hirohashi, S. ( 2002) Proc. Natl. Acad. Sci. U. S. A. 99, 365 - 370). The goal of this study was to determine whether FXYD5 can modulate the Na, KA-TPase activity, establish its cellular and tissue distribution, and characterize its biochemical properties. Anti-FXYD5 antibodies detected a 24-kDa polypeptide that was preferentially expressed in kidney, intestine, spleen, and lung. In kidney, FXYD5 resides in the basolateral membrane of the connecting tubule, the collecting tubule, and the intercalated cells of the collecting duct. However, there is also labeling of the apical membrane in long thin limb of Henle's loop. FXYD5 was effectively immunoprecipitated by antibodies to the alpha subunit of Na, K-ATPase and the anti-FXYD5 antibody immunoprecipitates alpha. Co-expressing FXYD5 with the alpha 1 and beta 1 subunits of the Na, K-ATPase in Xenopus oocytes elicited a more than 2-fold increase in pump activity, measured either as ouabain-blockable outward current or as ouabain-sensitive Rb-86(+) uptake. Thus, as found with other FXYD proteins, FXYD5 interacts with the Na, K-ATPase and modulates its properties.
