Receptor protein tyrosine kinase EphB4 is up-regulated in colon cancer.
Receptor protein tyrosine kinase EphB4 is up-regulated in colon cancer.
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DOI：
10.1186/1471-2199-2-15
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发表时间：
2001-01-01
影响因子：
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通讯作者：
Hardingham, J E
中科院分区：
文献类型：
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作者：
Stephenson, S A;Slomka, S;Hardingham, J E
背景技术背景：我们已经使用了市售的cDNA阵列，以确定EphB 4作为一个基因，是在结肠癌组织中上调时，与匹配的正常组织从同一patient.RESULTS：定量RT-PCR分析的EphB 4基因的表达表明，它的表达增加了82%的肿瘤样本相比，与匹配的正常组织从同一患者。使用免疫组织化学和Western分析技术与EphB 4特异性抗体，我们还表明，这种受体表达在上皮细胞的肿瘤组织和要么根本没有，或仅在低水平，在正常组织。本研究结果支持Eph受体在肿瘤形成中发挥作用的新观点，并表明进一步阐明该信号通路可能有助于识别肿瘤细胞的增殖。癌症治疗的靶点。
BACKGROUND: We have used commercially available cDNA arrays to identify EphB4 as a gene that is up-regulated in colon cancer tissue when compared with matched normal tissue from the same patient.RESULTS: Quantitative RT-PCR analysis of the expression of the EphB4 gene has shown that its expression is increased in 82% of tumour samples when compared with the matched normal tissue from the same patient. Using immunohistochemistry and Western analysis techniques with an EphB4-specific antibody, we also show that this receptor is expressed in the epithelial cells of the tumour tissue and either not at all, or in only low levels, in the normal tissue.CONCLUSION: The results presented here supports the emerging idea that Eph receptors play a role in tumour formation and suggests that further elucidation of this signalling pathway may identify useful targets for cancer treatment therapies.