Reduction of the surface-tension-lowering ability of surfactant after exposure to hypochlorous acid.
Reduction of the surface-tension-lowering ability of surfactant after exposure to hypochlorous acid.
复制标题
接触次氯酸后表面活性剂降低表面张力的能力降低。
DOI：
10.1042/bj2950019
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Cross,CE
中科院分区：
文献类型：
--
作者：
Merritt,TA;Amirkhanian,JD;Helbock,H;Halliwell,B;Cross,CE
活性物种次氯酸(HOCl/OCL-)是中性粒细胞呼吸爆发的主要产物。利用脉动表面张力测定仪，研究了HOCl/OCL-对人体表面活性物质及对表面活性物质Survanta、KL4和Exosurf的影响。HOCl/OCL可显著降低人表面活性物质的表面张力，且呈剂量依赖性。含有表面活性蛋白B和C(Survanta)的表面活性物质敏感性较低，而含有或不含有多肽的合成表面活性物质不受HOCl/OCL-(KL4，Exosurf)的影响。抗坏血酸和谷胱甘肽保护人表面活性物质免受HOCl/OC1-的失活。我们认为，肺泡室内活化的吞噬细胞所产生的HOCl/OCL-可损伤内源性表面活性物质，并影响外源性天然或其他表面活性物质的功能，特别是当肺内膜液中抗坏血酸和谷胱甘肽水平低于正常水平时，就像某些炎症性肺部疾病一样。
The reactive species hypochlorous acid (HOCl/OCl-) is a major product of the respiratory burst in activated neutrophils. We studied the effects of HOCl/OCl- on human surfactant and upon surfactants Survanta, KL4 and Exosurf, utilizing a pulsating surfactometer for measuring surface tension. HOCl/OCl- induced a marked dose-dependent decrease in the surface-tension-lowering activity of human surfactant. The surfactant containing surfactant proteins B and C (Survanta) was less sensitive; however, synthetic surfactants with or without peptides were not affected by HOCl/OCl- (KL4, Exosurf). Ascorbic acid and GSH protected human surfactant against inactivation by HOCl/OC1-. We suggest that HOCl/OCl- produced by activated phagocytes in the alveolar compartment of the lung could damage endogenous surfactant and affect the function of exogenously administered natural or other surfactants, especially if ascorbic acid and GSH levels in the lung lining fluids are subnormal, as is known to be the case in some inflammatory lung diseases.