Circulation of multiple genotypes of H1N2 viruses in a swine farm in Italy over a two-month period
Circulation of multiple genotypes of H1N2 viruses in a swine farm in Italy over a two-month period
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DOI：
10.1016/j.vetmic.2016.08.015
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发表时间：
2016-11-15
影响因子：
3.3
通讯作者：
Fusaro, Alice
中科院分区：
文献类型：
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作者：
Beato, Maria Serena;Tassoni, Luca;Fusaro, Alice
2012年8月，在意大利东北部的一个提供育肥仔猪的养殖场，一整年都记录了由甲型猪流感病毒（swIAV）引起的多次呼吸道疫情。该病毒最初是在该农场发现的，是一种重组的欧亚鸟样H1N1 （H1(av)N1）基因型，含有源自大流行H1N1 (a (H1N1)pdm09谱系的血凝素片段。为了控制感染，2013年11月在母猪中实施了针对甲型H1N1 pdm09、类人H1N2 （H1(hu)N2）、类人H3N2 （H3N2）和H1(av)N1病毒的疫苗接种计划。接种疫苗的母猪所生的35-75日龄仔猪，通过取样鼻腔拭子两个月来评估疫苗效力。对实施疫苗接种计划后纵向采集的8只猪流感病毒阳性鼻拭子进行全基因组测序，以研究病毒特征。在两个月的时间里，检测到涉及多个重配事件的两种不同的基因型。在如此短的时间内，多个重组基因型的意外循环凸显了猪流感病毒遗传多样性的复杂性，以及基于临床体征、流行病学数据和全基因组特征的更好的监测计划的必要性。(C) 2016 Elsevier B.V.版权所有
In August 2012 repeated respiratory outbreaks caused by swine influenza A virus (swIAV) were registered for a whole year in a breeding farm in northeast Italy that supplied piglets for fattening. The virus, initially characterized in the farm, was a reassortant Eurasian avian-like H1N1 (H1(av)N1) genotype, containing a haemagglutinin segment derived from the pandemic H1N1 (A(H1N1)pdm09) lineage. To control infection, a vaccination program using vaccines against the A(H1N1)pdm09, human-like H1N2 (H1(hu)N2), human-like H3N2 (H3N2), and H1(av)N1 viruses was implemented in sows in November 2013. Vaccine efficacy was assessed by sampling nasal swabs for two months in 35-75 day-old piglets born from vaccinated sows. Complete genome sequencing of eight swIAV-positive nasal swabs collected longitudinally from piglets after the implementation of the vaccination program was conducted to investigate the virus characteristics.Over the two-month period, two different genotypes involving multiple reassortment events were detected. The unexpected circulation of multiple reassortant genotypes in such a short time highlights the complexity of the genetic diversity of swIAV and the need for a better surveillance plan, based on the combination of clinical signs, epidemiological data and whole genome characterization. (C) 2016 Elsevier B.V. All rights reserved.