A Novel Protein-Based Anticancer Drug Encapsulating Nanosphere: Apoferritin-Doxorubicin Complex
A Novel Protein-Based Anticancer Drug Encapsulating Nanosphere: Apoferritin-Doxorubicin Complex
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DOI：
10.1166/jbn.2012.1406
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发表时间：
2012-06-01
影响因子：
2.9
通讯作者：
Calis, Sema
中科院分区：
文献类型：
--
作者：
Kilic, Mehmet A.;Ozlu, Erdal;Calis, Sema
开发一种均匀、生物相容性好、稳定且无毒的给药系统是抗癌药物给药策略中的一个具有挑战性的问题。铁蛋白是一种纳米大小的球形蛋白质，其内部有一个腔体，可以封装药物分子。载铁蛋白-阿霉素复合物是在阿霉素存在下通过“打开”和“关闭”载铁蛋白球体形成的。阿霉素包封采用直接和逐步改变溶液pH从2.5到7.4的方法。非变性聚丙烯酰胺凝胶表明，复合物的蛋白质笼成功地自组装成纳米球形式。结果发现，每个载铁蛋白最多可封装28个阿霉素分子，并且在头两天内不会发生明显的药物泄漏。载铁蛋白-阿霉素复合物是一种很有前途的抗癌药物纳米载体。
Developing a drug delivery system, which is uniform, biocompatible, stable and non-toxic, is a challenging issue in anticancer drug delivery strategies. Ferritin is a nano-size spherical protein with an internal cavity where drug molecules can be encapsulated. The apoferritin-doxorubicin complex has been formed by 'opening' and 'closing' the apoferritin sphere in the presence of doxorubicin. The doxorubicin encapsulation was carried out using direct and step-wise change of pH of the solution from 2.5 to 7.4. Non-denaturing polyacrylamide gels showed that the protein cage of the complex successfully self-assembles into its nanosphere form. It was found that up to 28 molecules of doxorubicin can be capsulated per apoferritin protein and no significant drug leakage occurs during the first two days. The apoferritin-doxorubicin complex is a promising nanocarrier for the delivery of anticancer drugs.