The Plasmodium vivax Pv41 surface protein: Identification and characterization
The Plasmodium vivax Pv41 surface protein: Identification and characterization
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DOI：
10.1016/j.bbrc.2008.10.129
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发表时间：
2008-12-26
影响因子：
3.1
通讯作者：
Patarroyo, Manuel A.
中科院分区：
文献类型：
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作者：
Angel, Diana I.;Monguia, Alvaro;Patarroyo, Manuel A.
最近，在中美洲和地中海报告的近81%的疟疾病例与间日疟原虫有关。由于间日疟原虫在体外培养的困难，大多数关于间日疟原虫的研究都集中在通过与恶性疟原虫候选疫苗蛋白的同源性比较来鉴定新的抗原上。在这项研究中，我们利用网络生物信息学数据库、分子技术和免疫化学分析方法，在间日疟原虫中鉴定并表征了Pf41同源物，因此命名为Pv41。Pv41蛋白是一种384个氨基酸长的抗原，由单个外显子编码，具有配子体表面蛋白特征的两个s48/45结构域。我们还证实了Pv41在红细胞内寄生虫后期的转录和表达，并确定了其在寄生虫表面的亚细胞定位。(C) 2008爱思唯尔公司版权所有。
Recently, Plasmodium vivax has been related to nearly 81% of malaria cases reported in Central America and the Mediterranean. Due to the difficulty of culturing this parasite species in vitro, most studies on P. vivax have focused on the identification of new antigens by homology comparison with P. falciparum vaccine candidate proteins. In this study, we have identified and characterized a Pf41 homologue in P. vivax, hence named Pv41, by following Such approach and using web-available bioinformatics databases, molecular techniques and immunochemistry assays. Pv41 protein is a 384-amino-acid-long antigen encoded by a single exon that exhibits two s48/45 domains characteristic of gametocyte Surface proteins. We have also demonstrated Pv41 transcription and expression during late intra-erythrocytic parasite stages and defined its subcellular localization on the parasite surface. (C) 2008 Elsevier Inc. All rights reserved.