Evaluation and simplified measurement of infarct size by myocardial contrast echocardiography in a rat model of myocardial infarction
Evaluation and simplified measurement of infarct size by myocardial contrast echocardiography in a rat model of myocardial infarction
复制标题
心肌梗死大鼠模型中心肌造影超声心动图对梗死面积的评估和简化测量
DOI：
10.1007/s10554-009-9474-x
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发表时间：
2009-10-01
影响因子：
2.1
通讯作者：
Liu, Yili
中科院分区：
文献类型：
--
作者：
Chen, Xianghui;Cui, Kai;Liu, Yili
探讨心肌声学造影（MCE）预测大鼠心肌梗死（MI）模型梗死面积（IS）的可行性和准确性，并比较简化的单平面法和传统的三平面法。50只雄性SD大鼠行左前降支结扎术，于MI后8h行MCE检查。采用单平面法和三平面法计算IS，并与氯化三苯基四氮唑（TTC）染色法进行比较。简化单平面MCE法和TTC法的IS值相似（38.48 ± 16.80%vs.35.72 ± 15.33%，P> 0.05），且呈良好的正相关（r= 0.851，P < 0.001）。简化单平面法与常规MCE法测得的IS值也呈高度显著相关（r= 0.973，P < 0.001）。Bland-Altman图也显示出令人满意的一致性。MCE被证实是一种新的技术来量化心肌梗死大鼠的梗死面积。中乳头肌水平的单次测量可能成为一种简单、有效和可靠的体内IS评估方法。
To test the feasibility and accuracy of myocardial contrast echocardiography (MCE) for predicting infarct size (IS) in a rat model of myocardial infarction (MI) and to compare a simplified single plane-based measurement of IS with the conventional three plane-based approach. Fifty male SD rats underwent left anterior descending artery ligation and were evaluated by MCE 8 h post MI. IS was calculated by the single and three plane-based approaches, compared to that determined by triphenyltetrazolium chloride (TTC) staining method. Simplified single plane-based MCE approach and TTC method showed similar IS values (38.48 ± 16.80% vs. 35.72 ± 15.33%,P> 0.05) and presented a favorable positive correlation (r= 0.851,P< 0.001). IS values derived from simplified single plane-based approach was also highly significantly correlated with that by the conventional MCE method (r= 0.973,P< 0.001). Bland–Altman plots also displayed satisfactory agreement between them. MCE was validated as a novel technique to quantify infarct area in rats with MI. A single measurement at the mid-papillary muscle level may become a simple, efficient and reliable approach for in vivo IS assessment.