The mass mortality of blue mussels (Mytilus spp.) from the Atlantic coast of France is associated with heavy genomic abnormalities as evidenced by flow cytometry
The mass mortality of blue mussels (Mytilus spp.) from the Atlantic coast of France is associated with heavy genomic abnormalities as evidenced by flow cytometry
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DOI：
10.1016/j.jip.2016.06.001
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发表时间：
2016-07-01
影响因子：
3.4
通讯作者：
Ledu, Christophe
中科院分区：
文献类型：
--
作者：
Benabdelmouna, Abdellah;Ledu, Christophe
自2014年以来，法国的贻贝产业一直面临严重的死亡疫情(90%-100%)，对青少年和成年人都有影响。这份报告提供了与来自法国大西洋海岸的蓝贻贝(Mytilus edulis-gallagencialis)死亡暴发有关的严重基因组异常的证据。在这项研究中，用流式细胞术(FCM)研究了白血病细胞的倍性特征，揭示了从亚二倍体到四倍体的不寻常的、广泛的倍性分布。此外，FCM还用于在个体和群体水平上使用非二倍体核的百分比来评估不同的基因组异常阈值(GA%)。当处于S-G2/M期的血细胞超过10%时，个别贻贝被认为是异常的。在种群水平上，提出了平均异常强度的阈值为6%，这意味着在种群中，超过6%的单个贻贝的血细胞必须超过10%处于S-G2/M期。GA%被发现对最终死亡率有显著的预测作用。根据建立的阈值，只有两个研究分析的贻贝种群被认为具有良好的细胞遗传学质量，而所有其他种群似乎都受到了影响。FCM提供了一个非常强大的工具来帮助管理法国目前的蓝贻贝死亡率。我们还认为，每年广泛地测定野生和栽培贻贝的细胞遗传学质量，以及独家使用FCM合格的贻贝种子，应该是优先事项。(C)2016 Elsevier Inc.保留所有权利。
Since 2014, France's blue mussel industry has been facing heavy mortality outbreaks (90-100%) affecting both juveniles and adults. This report presents evidence of heavy genomic abnormalities associated with mortality outbreaks in blue mussels, Mytilus edulis-galloprovincialis, from the Atlantic coast of France. In this study, ploidy characteristics of hemic cells were investigated using Flow CytoMetry (FCM), revealing an unusual, broad continuum of ploidy distribution from hypodiploidy to tetraploidy. FCM was additionally used to evaluate, at individual and populations levels, different thresholds of genomic abnormality (GA%) using the percentage of non-diploid nuclei. Individual mussels were considered to be abnormal when more than 10% of hemocytes in S-G2/M phase were present. At the population level, a threshold of 6% for the mean intensity of the abnormality is proposed, which means in the population, more than 6% of individual mussels have to present with more than 10% of their hemocytes in S-G2/M phase. GA% was found to be significantly predictive of the final mortality. Based on the established thresholds, only two mussel stocks analyzed in this study were considered to have good cytogenetic quality, while all other stocks appeared to be affected. FCM offers a very powerful tool to help manage current blue mussel mortality in France. We also believe that annual and extensive determination of cytogenetic quality of wild and cultivated mussel beds along with exclusive use of FCM-qualified mussel seeds should be a priority. (C) 2016 Elsevier Inc. All rights reserved.