Interleukin-21 mRNA expression during virus infections
Interleukin-21 mRNA expression during virus infections
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DOI：
10.1016/j.cyto.2005.11.014
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发表时间：
2006-01-07
期刊：
影响因子：
3.8
通讯作者：
Hokland, M
中科院分区：
文献类型：
--
作者：
Holm, C;Nyvold, CG;Hokland, M
白细胞介素-21是一种对先天免疫系统和适应性免疫系统中活化白细胞的增殖和分化具有深远影响的细胞因子。在体外实验中，抗原激活诱导CD4+ T细胞产生IL-21。在体内免疫应答中，IL-21对不同白细胞的增殖和激活作用在何时、何地以及如何发挥作用，迄今尚未得到充分研究。我们首次在体内证明，当小鼠受到单纯疱疹病毒2型（HSV-2）或淋巴细胞性脉络丛脑膜炎病毒（LCMV）攻击时，脾脏会产生IL-21 mRNA。我们在HSV-2攻击小鼠中发现，这种产生发生在CD4+ T细胞片段中，而在CD4+ T细胞缺失片段中不存在。我们还发现，在HSV-2和lcmv攻击小鼠中，IL-21 mRNA产生的峰值与适应性免疫反应的发生一致。因此，我们的数据表明IL-21在针对病毒感染的适应性免疫反应的早期阶段发挥作用。(c) 2005 Elsevier Ltd版权所有。
Interleukin-21 is a cytokine with profound impact on the proliferation and differentiation of activated leukocytes of both the innate and adaptive immune system. In experiments in vitro, antigen activation induces IL-21 production in CD4+ T cells. Where, when, and how the proliferative and activational effects of IL-21 on different leukocytes come into play in vivo in an immune response has so far not been fully investigated. We show here for the first time in vivo, that IL-21 mRNA is produced in the spleen when mice are challenged with herpes simplex virus type 2 (HSV-2) or lymphocytic choriomeningitis virus (LCMV). We show in HSV-2 challenged mice that this production takes place in CD4+ T cell fractions and is absent in CD4+ T cell-depleted fractions. We also show that the peak of IL-21 mRNA production in both the HSV-2 and LCMV-challenged mice coincides with the onset of the adaptive immune response. Thus, our data suggest a role for IL-21 in the early stages of adaptive immune response against virus infections. (c) 2005 Elsevier Ltd. All rights reserved.