Novel dummy molecularly imprinted polymers for matrix solid-phase dispersion extraction of eight fluoroquinolones from fish samples.
Novel dummy molecularly imprinted polymers for matrix solid-phase dispersion extraction of eight fluoroquinolones from fish samples.
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DOI：
10.1016/j.chroma.2014.07.007
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发表时间：
2014-09
期刊：
影响因子：
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通讯作者：
Xiaoli Sun;Jincheng Wang;Yun Li;Jiajia Yang;Jing Jin;Syed Mazhar Shah;Jiping Chen
中科院分区：
文献类型：
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作者：
Xiaoli Sun;Jincheng Wang;Yun Li;Jiajia Yang;Jing Jin;Syed Mazhar Shah;Jiping Chen
制备了一系列新型的虚拟分子印迹聚合物（DMIPs），作为氟喹诺酮类药物的高类别选择性吸附剂。首次使用无毒的假模板大豆苷元在合成的聚合物中建立了氟喹诺酮类药物的特异性分子识别位点。研究了致孔剂极性对虚拟分子印迹效应的影响。使用二甲基亚砜-乙腈（1：1.8，v/v）作为致孔剂制备的DMIP实现了氟喹诺酮类的最高印迹因子（IF），范围为IF 13.4-84.0。然后，基于虚拟分子印迹基质固相分散（DMI-MSPD），该DMIP用于从鱼样品中选择性提取八种氟喹诺酮类药物（氟罗沙星、氧氟沙星、诺氟沙星、培氟沙星、环丙沙星、洛美沙星、恩诺沙星和加替沙星）。随后通过配备有荧光检测器（FLD）的高效液相色谱（HPLC）分析提取的氟喹诺酮类药物。所开发的方法具有可接受的回收率（64.4 - 102.7%）和精密度（RSD：1.7 - 8.5%，n = 5），用于测定鱼样品中的氟喹诺酮类药物在10和100 ng g-1水平。8种氟喹诺酮类药物的检出限（LOD）在0.06 - 0.22 ng g−1之间。结果表明，优化的样品前处理方法在鱼类样品中痕量氟喹诺酮类药物的常规分析中具有很大的潜力。
A series of novel dummy molecularly imprinted polymers (DMIPs) were prepared as highly class-selective sorbents for fluoroquinolones. A non-poisonous dummy template, daidzein, was used for the first time to create specific molecular recognition sites for fluoroquinolones in the synthesized polymers. The influence of porogen polarity on dummy molecular imprinting effect was studied. The DMIP prepared using dimethylsulfoxide−acetonitrile (1:1.8, v/v) as porogen achieved the highest imprinting factors (IF) for fluoroquinolones over a range of IF 13.4–84.0. This DMIP was then used for selective extraction of eight fluoroquinolones (fleroxacin, ofloxacin, norfloxacin, pefloxacin, ciprofloxacin, lomefloxacin, enrofloxacin and gatifloxacin) from fish samples based on dummy molecularly imprinted matrix solid-phase dispersion (DMI-MSPD). The extracted fluoroquinolones were subsequently analyzed by high-performance liquid chromatography (HPLC) equipped with a fluorescence detector (FLD). The developed method had acceptable recoveries (64.4−102.7%) and precision (RSDs: 1.7−8.5%,n= 5) for determination of fluoroquinolones in fish samples fortified at levels of 10 and 100 ng g−1. The limits of detection (LODs) for identification of eight fluoroquinolones ranged between 0.06 and 0.22 ng g−1. The results demonstrated great potential of the optimized method for sample preparation in routine analysis of trace fluoroquinolones in fish samples.