STRUCTURE AND DYNAMICS OF THE FENESTRAE-ASSOCIATED CYTOSKELETON OF RAT-LIVER SINUSOIDAL ENDOTHELIAL-CELLS
STRUCTURE AND DYNAMICS OF THE FENESTRAE-ASSOCIATED CYTOSKELETON OF RAT-LIVER SINUSOIDAL ENDOTHELIAL-CELLS
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DOI：
10.1002/hep.1840210130
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发表时间：
1995-01-01
期刊：
影响因子：
13.5
通讯作者：
WISSE, E
中科院分区：
文献类型：
--
作者：
BRAET, F;DEZANGER, R;WISSE, E
本文描述了大鼠肝窦内皮细胞与窗孔和筛板相关的细胞骨架。窗孔控制着血液和实质细胞之间的交换。我们提出的证据表明，几种改变隔板和筛板的试剂也会引起细胞骨架的变化。将培养的肝内皮细胞稍作固定，用细胞骨架提取缓冲液处理。洗涤剂提取的培养细胞整装制备成扫描电子显微镜(SEM)或透射电子显微镜(TEM)。提取的细胞显示一个完整的、错综复杂的细胞骨架；筛板和窗孔由细胞骨架元素描绘。窗孔周围是丝状的、与窗孔相关的细胞骨架，平均纤丝厚度为16 nm。筛板被微管包围和描绘，微管与额外的分枝细胞骨架元件一起形成一个网络。在培养细胞中加入乙醇，这些窗孔相关细胞骨架环的直径增加了5%，而5-羟色胺处理使直径减少了20%。这些观察表明，窗孔相关的细胞骨架可能在不同的处理后改变了窗孔的大小。细胞松弛素B处理后，窗孔数目增加。然而，细胞松弛素B没有改变窗孔相关细胞骨架环的结构，而是分散了微管。总之，晶状体上皮细胞有一个细胞骨架，它定义和支撑筛板和窗孔。窗孔相关细胞骨架是一种动态结构，在不同处理后对窗孔的大小起着维持和调节的作用。因此，窗孔相关的细胞骨架控制着重要的肝内皮细胞滤过功能。
This article describes the cytoskeleton associated with fenestrae and sieve plates of rat liver sinusoidal endothelial cells. Fenestrae control the exchange between the blood and parenchymal cells. We present evidence indicating that several agents that change the fenestrae and sieve plates also cause changes in the cytoskeleton. Cultured liver endothelial cells (LECs) were slightly fixed and treated with cytoskeleton extraction buffer. Detergent-extracted whole mounts of cultured cells were prepared for either scanning electron microscopy (SEM) or transmission electron microscopy (TEM). Extracted cells show an integral intricate cytoskeleton; sieve plates and fenestrae are delineated by cytoskeleton elements. Fenestrae are surrounded by a filamentous, fenestrae-associated cytoskeleton with a mean filament thickness of 16 nm. Sieve plates are surrounded and delineated by microtubuli, which form a network together with additional branching cytoskeletal elements. The addition of ethanol to cultured cells enlarged the diameter for these fenestrae-associated cytoskeleton rings by 5%, whereas serotonin treatment reduced the diameter by 20%. These observations indicate that the fenestrae-associated cytoskeleton probably changes the size of fenestrae after different treatments. After treatment with cytochalasin B the number of fenestrae increased. However, cytochalasin B did not change the structure of the fenestrae-associated cytoskeleton ring, but disperses the microtubuli. In conclusion, LECs have a cytoskeleton that defines and supports sieve plates and fenestrae. Fenestrae-associated cytoskeleton is a dynamic structure and plays a role in maintaining and regulating the size of fenestrae after different treatments. Therefore, the fenestrae-associated cytoskeleton controls the important hepatic function of endothelial filtration.