Expression patterns of nestin and dentin sialoprotein during dentinogenesis in mice
Expression patterns of nestin and dentin sialoprotein during dentinogenesis in mice
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DOI：
10.2220/biomedres.33.119
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发表时间：
2012-04-01
影响因子：
1.2
通讯作者：
Ohshima, Hayato
中科院分区：
文献类型：
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作者：
Quispe-Salcedo, Angela;Ida-Yonemochi, Hiroko;Ohshima, Hayato
分化的成牙本质细胞不能通过一个独特的表型标记物来识别，但是牙本质磷蛋白（Dpp）、牙本质唾液蛋白（Dsp）、牙本质基质蛋白1（Dmp 1）和巢蛋白的表达的组合可能对这些细胞的评估有价值。然而，使用这些蛋白质的发现仍然存在争议。本研究旨在比较两种成牙本质细胞分化标志物nestin和Dsp在小鼠牙本质形成过程中的作用。我们进行了免疫组织化学和/或原位杂交技术巢蛋白和Dsp使用3周龄的门牙，以及出生后1天至8周龄的磨牙。前成牙本质细胞开始表达巢蛋白和Dsp蛋白和Dsp mRNA，根据成牙本质细胞分化在切牙和发育磨牙的进展，其强度增加。巢蛋白在分化的成牙本质细胞中持续表达，即使在牙本质基质沉积完成后也是如此。Dsp mRNA的表达与成牙本质细胞分泌牙本质基质的活动相一致。除分化的成牙本质细胞外，其他牙髓细胞、前牙本质基质和牙本质小管也呈Dsp阳性反应。总之，巢蛋白作为成牙本质细胞的分化标志物是有价值的，而Dsp mRNA是其分泌活性的功能标志物。
Differentiated odontoblasts could not be identified by one unique phenotypic marker, but the combination of expression of dentin phosphoprotein (Dpp), dentin sialoprotein (Dsp), dentin matrix protein 1 (Dmp1), and nestin may be valuable for the assessment of these cells. However, the findings using these proteins remain controversial. This study aimed to compare two odontoblast differentiation markers: nestin and Dsp in the process of dentinogenesis in mice. We performed immunohistochemistry and/or in situ hybridization technique for nestin and Dsp using 3-week-old incisors as well as postnatal 1-day- to 8-week-old molars. Preodontoblasts began to express nestin and Dsp proteins and Dsp mRNA, which increased in their intensity according to the progress of odontoblast differentiation in both incisors and developing molars. Nestin was consistently expressed in the differentiated odontoblasts even after the completion of dentin matrix deposition. The expression of Dsp mRNA coincided with the odontoblast secretory activity for dentin matrix deposition. In contrast, other pulpal cells, predentin matrix and dentinal tubules also showed a positive reaction for Dsp protein in addition to differentiated odontoblasts. In conclusion, nestin is valuable as a differentiation marker for odontoblasts, whereas Dsp mRNA is a functional marker for their secretory activity.