Identification of microtubule-associated proteins in the meiotic spindle of surf clam oocytes.
Identification of microtubule-associated proteins in the meiotic spindle of surf clam oocytes.
复制标题
在冲浪蛤卵母细胞的减数分裂纺锤体中鉴定微管相关蛋白。
DOI：
10.1083/jcb.84.2.235
复制
发表时间：
1980-02
影响因子：
7.8
通讯作者：
Murphy, D B
中科院分区：
文献类型：
--
作者：
Murphy, D B
从海蛤卵母细胞分离到形态学纯度的减数分裂纺锤体是生物化学复杂的，由许多多肽组成。这些蛋白质分为两类：（a）多肽，其显然是细胞质蛋白质并且不与纺锤体特异性相关;和（B）多肽，其与纺锤体特异性相关。纺锤体相关蛋白的一个子集，包括一个250，000摩尔重量的组分，通过冷解聚和热聚合的循环与纺锤体微管蛋白保持在一起，表明它们是微管相关蛋白。
Meiotic spindles isolated from surf clam oocytes to morphological purity are biochemically complex, consisting of many polypeptides. These proteins fall into two classes: (a) polypeptides that are apparently cytoplasmic proteins and are not specifically associated with the spindle; and (b) polypeptides that are specifically associated with the spindle. A subset of the spindle-associated proteins, including a 250,000 mol wt component, remain with spindle tubulin through cycles of cold depolymerization and warm polymerization, showing that they are microtubule-associated proteins.