LC-MS/MS Profiling-Based Secondary Metabolite Screening of Myxococcus xanthus
LC-MS/MS Profiling-Based Secondary Metabolite Screening of Myxococcus xanthus
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DOI：
10.4014/jmb.0711.775
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发表时间：
2009-01-01
影响因子：
2.8
通讯作者：
Lee, Choong Hwan
中科院分区：
文献类型：
--
作者：
Kim, Jiyoung;Choi, Jung Nam;Lee, Choong Hwan
粘杆菌，革兰氏阴性土壤细菌，是一种众所周知的生物活性次生代谢物的生产者。因此，本研究提出了一种高通量筛选4株野生型黄粘球菌次级代谢物的方法学方法。首先，使用细胞外粗提取物进行电喷雾电离质谱分析（ESI-MS）。结果，检测到22个代谢物峰，然后使用m/z值，保留时间和MS/MS碎片模式分析进行代谢物谱分析。在这些峰中，有一个未知的化合物峰被鉴定为类似于myxalamid A， B和C系列。串联质谱分析和质谱分析证实myxalamid K是一种从黄原草中分离得到的新化合物。综上所述，基于LC-MS/ ms的多种次生代谢物化学筛选似乎是发现未知微生物次生代谢物的有效方法。
Myxobacteria, Gram-negative soil bacteria, are a well-known producer of bioactive secondary metabolites. Therefore, this study presents a methodological approach for the high-throughput screening of secondary metabolites from 4 wild-type Myxococcus xanthus strains. First, electrospray ionization mass spectrometry (ESI-MS) was performed using extracellular crude extracts. As a result, 22 metabolite peaks were detected, and the metabolite profiling was then conducted using the m/z value, retention time, and MS/MS fragmentation pattern analyses. Among the peaks, one unknown compound peak was identified as analogous to the myxalamid A, B, and C series. An analysis of the tandem mass spectrometric fragmentation patterns and HR-MS identified myxalamid K as a new compound derived from M. xanthus. In conclusion, LC-MS/MS-based chemical screening of diverse secondary metabolites would appear to be an effective approach for discovering unknown microbial secondary metabolites.