FIBRONECTIN BENEATH REEPITHELIALIZING EPIDERMIS INVIVO - SOURCES AND SIGNIFICANCE
FIBRONECTIN BENEATH REEPITHELIALIZING EPIDERMIS INVIVO - SOURCES AND SIGNIFICANCE
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DOI：
10.1111/1523-1747.ep12537020
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发表时间：
1983-01-01
影响因子：
6.5
通讯作者：
COLVIN, RB
中科院分区：
文献类型：
--
作者：
CLARK, RAF;WINN, HJ;COLVIN, RB
纤连蛋白和纤维蛋白原在切除伤口的上皮再生过程中发生在迁移的表皮舌下，并且纤连蛋白在伤口愈合过程中与毛细血管和成纤维细胞向内生长一起增加。虽然纤连蛋白是由血管增殖产生的，但与上皮再生和成纤维细胞向内生长相关的纤连蛋白的来源尚未确定。表皮下纤连蛋白主要来源于切除伤口上皮再形成早期的血浆，并且来源于上皮再形成晚期的血浆和原位产生。这一发现是通过从免疫抑制小鼠侧腹上愈合良好的大鼠异种移植物的中心摘除3 mm皮肤，在损伤后2、4、7和10天收获开放性伤口部位，并用与荧光素缀合的相互种特异性抗纤连蛋白抗体对标本进行染色来确定的。在第一个4天受伤后，新形成的大鼠表皮迁移主要是小鼠纤维连接蛋白。切除后7天，大鼠表皮在含有小鼠和大鼠纤连蛋白或仅含有大鼠纤连蛋白的基质上转移，表明纤连蛋白的主要组分是原位产生的。虽然这些观察结果的生物学意义尚未完全阐明，纤连蛋白可能是临时基质的一部分，其功能是支持（如果不积极参与）细胞募集到炎症或伤口愈合部位。
Fibronectin and fibrinogen occur under the migrating epidermal tongue during reepithelialization of an excisional wound, and fibronectin increases in conjunction with capillary and fibroblast ingrowth during wound healing. Although fibronectin is produced by proliferating blood vessels, the source of fibronectin associated with reepithelialization and fibroblast ingrowth has not been determined. Subepidermal fibronectin derives mostly from plasma early in reepithelialization of an excisional wound and comes from both plasma and in situ production late in reepithelialization. This finding was established by extirpating 3 mm of skin from the center of a well-healed rat xenograph on the flanks of immunosuppressed mice, harvesting the open wound sites at 2, 4, 7 and 10 days after injury, and stainng the specimens with reciprocal species-specific anti-fibronectin antibodies conjugated with fluorescein. In the first 4 days after wounding, newly forming rat epidermis migrated mainly over mouse fibronectin. By 7 days after excision, the rat epidermis transits over a matrix containing both mouse and rat fibronectin, or rat fibronectin alone, indicating that a major component of the fibronectin is produced in situ. Although the biological significance of these observations has not been fully elucidated, fibronectin may be part of a provisional matrix that functions to support, if not actively participate in, cell recruitment to sites of inflammation or wound healing.