Skeletal nutrient vascular adaptation induced by external oscillatory intramedullary fluid pressure intervention.
Skeletal nutrient vascular adaptation induced by external oscillatory intramedullary fluid pressure intervention.
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DOI：
10.1186/1749-799x-5-18
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发表时间：
2010-03-11
影响因子：
2.6
通讯作者：
Qin YX
中科院分区：
文献类型：
--
作者：
Lam H;Brink P;Qin YX
由负荷引起的间质液流动已被证明是调节骨量和形态的重要介质。结果表明，髓内压力（ImP）产生的流体运动提供了一个源的压力梯度在骨。这种动态ImP可以改变邻近骨并直接连接到骨髓腔的营养血管内的血流，进一步启动营养血管适应。假设振荡性ImP可以介导骨骼营养血管中的血流并触发血管重构。本研究的目的是评价动态ImP刺激诱导的血管重塑作为ImP频率的函数。使用禽类模型，将动力学生理流体ImP（70 mmHg，峰-峰）以3 Hz或30 Hz施加于左尺骨的骨髓腔中，10分钟/天，5天/周，持续3或4周。对主要滋养动脉进行组织形态计量学测量，以量化动脉壁面积、管腔面积、壁厚度和平滑肌细胞层数，以进行比较。初步结果表明，急性周期性ImP刺激可显著增加滋养动脉管壁面积达50%，管壁厚度达20%，平滑肌细胞层数达37%。此外，3周的急性刺激足以改变动脉结构特性，即，动脉壁面积的增加，而4周的负荷，无论负荷频率只有最小的变化。这些数据表明了血管适应和应用ImP改变之间相互关系的潜在机制。急性ImP可能会引发骨营养血管的重建，最终可能会改变骨的血液供应。
Interstitial fluid flow induced by loading has demonstrated to be an important mediator for regulating bone mass and morphology. It is shown that the fluid movement generated by the intramedullary pressure (ImP) provides a source for pressure gradient in bone. Such dynamic ImP may alter the blood flow within nutrient vessel adjacent to bone and directly connected to the marrow cavity, further initiating nutrient vessel adaptation. It is hypothesized that oscillatory ImP can mediate the blood flow in the skeletal nutrient vessels and trigger vasculature remodeling. The objective of this study was then to evaluate the vasculature remodeling induced by dynamic ImP stimulation as a function of ImP frequency. Using an avian model, dynamics physiological fluid ImP (70 mmHg, peak-peak) was applied in the marrow cavity of the left ulna at either 3 Hz or 30 Hz, 10 minutes/day, 5 days/week for 3 or 4 weeks. The histomorphometric measurements of the principal nutrient arteries were done to quantify the arterial wall area, lumen area, wall thickness, and smooth muscle cell layer numbers for comparison. The preliminary results indicated that the acute cyclic ImP stimuli can significantly enlarge the nutrient arterial wall area up to 50%, wall thickness up to 20%, and smooth muscle cell layer numbers up to 37%. In addition, 3-week of acute stimulation was sufficient to alter the arterial structural properties, i.e., increase of arterial wall area, whereas 4-week of loading showed only minimal changes regardless of the loading frequency. These data indicate a potential mechanism in the interrelationship between vasculature adaptation and applied ImP alteration. Acute ImP could possibly initiate the remodeling in the bone nutrient vasculature, which may ultimately alter blood supply to bone.