Sensitive detection of MCF-7 human breast cancer cells by using a novel DNA-labeled sandwich electrochemical biosensor
Sensitive detection of MCF-7 human breast cancer cells by using a novel DNA-labeled sandwich electrochemical biosensor
复制标题
使用新型 DNA 标记夹心电化学生物传感器灵敏检测 MCF-7 人乳腺癌细胞
DOI：
10.1016/j.bios.2018.09.062
复制
发表时间：
2018-12-30
影响因子：
12.6
通讯作者：
Chen, Mei
中科院分区：
文献类型：
--
作者：
Yang, Yuhan;Fu, Yongyao;Chen, Mei
简单、快速、灵敏、特异的癌细胞检测对癌症的诊断和预后起着举足轻重的作用。我们开发了一种新型的dna标记三明治电化学生物传感器，该传感器基于3D石墨烯修饰的玻璃碳电极和Au纳米笼(Au NCs)/氨基功能化多壁碳纳米管（MWCNT-NH2）的混合物，用于通过差分脉冲伏安法对MCF-7乳腺癌细胞进行无标记和选择性检测。利用扫描电镜和电化学方法（包括循环伏安法和电化学阻抗谱）研究了Au NCs/ mwcnts - nh2基生物传感器的逐层组装和细胞检测性能。由于dna标记抗体和基于纳米材料的信号放大策略的优势，所制备的细胞传感器在检测1.0 × 10(2) ~ 1.0 × 10(6)个细胞mL（-1）范围内具有很高的特异性和灵敏度，检测限低至80个细胞mL(-1) （3 sigma/斜率）。此外，该生物传感器在检测MCF-7细胞时表现出高选择性，显示出相当大的实际应用潜力。提出的dna标记三明治电化学生物传感器提供了一种稳定、灵敏的检测癌细胞的方法，在癌症诊断方面具有潜在的应用前景。
The simple, rapid, sensitive, and specific detection of cancer cells plays a pivotal role in the diagnosis and prognosis of cancer. We developed a novel DNA-labeled sandwich electrochemical biosensor based on a glassy carbon electrode modified with 3D graphene and a hybrid of Au nanocages (Au NCs)/amino-functionalized multiwalled carbon nanotubes (MWCNT-NH2) for label-free and selective detection of MCF-7 breast cancer cells via differential pulse voltammetry. The layer-by-layer assembly and cell-detection performance of the Au NCs/MWCNTs-NH2-based biosensor were investigated using scanning electron microscopy and electrochemical methods including cyclic voltammetry and electrochemical impedance spectroscopy. Owing to the advantages of DNA-labeled antibodies and a nanomaterial-based signal amplification strategy, the fabricated cytosensor exhibited high specificity and sensitivity when detecting MCF-7 cells in the range of 1.0 x 10(2) to 1.0 x 10(6) cells mL(-1) with a low detection limit of 80 cells mL(-1) (3 sigma/slope). Furthermore, the biosensor exhibited high selectivity when detecting MCF-7 cells and showed considerable potential for practical applications. The proposed DNA-labeled sandwich electrochemical biosensor provides a stable, sensitive approach to detecting cancer cells and is promising in terms of potential applications to cancer diagnosis.