THE ACTIVATION PROCESS OF ARABIDOPSIS-THALIANA A1-GENE ENCODING THE TRANSLATION ELONGATION-FACTOR EF-1-ALPHA IS CONSERVED AMONG ANGIOSPERMS
THE ACTIVATION PROCESS OF ARABIDOPSIS-THALIANA A1-GENE ENCODING THE TRANSLATION ELONGATION-FACTOR EF-1-ALPHA IS CONSERVED AMONG ANGIOSPERMS
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DOI：
10.1007/bf00047711
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发表时间：
1992-04-01
影响因子：
5.1
通讯作者：
LESCURE, B
中科院分区：
文献类型：
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作者：
CURIE, C;LIBOZ, T;LESCURE, B
在拟南芥中，Al EF-1-α基因的激活过程取决于几个元件。使用GUS报告基因，瞬时表达实验已经表明，Al启动子的上游顺式作用元件的突变，或位于5'非编码区内的内含子的缺失，类似地影响双子叶植物或单子叶植物原生质体中的表达。本文报道的结果强烈表明，该5'内含子在玉米中正确剪接。我们发现，两个反式作用因子，特别是与上游激活序列（TEF 1盒）相互作用，存在于从A。thaliana，Brassica rapa，Nicotiana tabacum和Z.梅斯此外，在番茄EF-1-α启动子内的大致相同位置发现的与TEF 1盒同源的DNA序列与相同的反式作用因子相互作用。与A. thaliana和L.番茄TEF 1盒序列使我们能够确定影响与相应反式作用因子相互作用的该上游元件的突变。这些结果支持了A.拟南芥EF-1-α基因在被子植物中是保守的，并提供了关于TEF 1盒功能结构的有趣数据。
In Arabidopsis thaliana, the activation process of the Al EF-1-alpha-gene depends on several elements. Using the GUS reporter gene, transient expression experiments have shown that mutations of upstream cis-acting elements of the Al promoter, or the deletion of an intron located within the 5' non-coding region, similarly affect expression in dicot or monocot protoplasts. The results reported here strongly suggest that this 5' intron is properly spliced in Zea mays. We show that two trans-acting factors, specifically interacting with an upstream activating sequence (the TEF 1 box), are present in nuclear extracts prepared from A. thaliana, Brassica rapa, Nicotiana tabacum and Z. mays. In addition, a DNA sequence homologous to the TEF 1 box, found at approximately the same location within a Lycopersicon esculentum EF-1-alpha-promoter, interacts with the same trans-acting factors. Homologies found between the A. thaliana and L. esculentum TEF 1 box sequences have allowed us to define mutations of this upstream element which affect the interaction with the corresponding trans-acting factors. These results support the notion that the activation processes of A. thaliana EF-1-alpha-genes have been conserved among angiosperms and provide interesting data on the functional structure of the TEF 1 box.