Isolation of Plastid Ribosomes
Isolation of Plastid Ribosomes
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质体核糖体的分离
DOI：
10.1007/978-1-4939-6533-5_20
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
Yamaguchi K
中科院分区：
文献类型：
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作者：
永島宗弥・藪本真央（高知大農）・HAEKYUN YOO（北大院水産）・池田譲（琉球大理）;上田幸男（農林水産総合技術支援セ）;桜井 泰憲（北大院水産）・足立亨介・森岡克司（高知大農）;Yamaguchi K
质体核糖体负责植物叶片、绿色藻类细胞中的大部分蛋白质合成，以及红藻的菌体中的绝大多数蛋白质合成。质体翻译不仅是光合作用所必需的，也是植物和藻类发育/分化所必需的。虽然一些分离的质体核糖体从几个绿色谱系的特点是生化和蛋白质组学的方法，深入的蛋白质组学，包括分析翻译后修饰和加工，比较蛋白质组学的质体核糖体分离的细胞在不同的条件下生长，和那些从不同的类群仍有待进行。从更广泛的物种中分离纯化质体核糖体的方法的建立将有利于研究质体核糖体的结构、功能和进化之间的关系。在这里，我描述的方法，并提供示例协议提取和分离的质体核糖体从单细胞绿色衣藻（衣藻reinhardtii），陆地植物（拟南芥），和海洋红色macrophora（Pyropia yezoensis）。
Plastid ribosomes are responsible for a large part of the protein synthesis in plant leaves, green algal cells, and the vast majority in the thalli of red algae. Plastid translation is necessary not only for photosynthesis but also for development/differentiation of plants and algae. While some isolated plastid ribosomes from a few green lineages have been characterized by biochemical and proteomic approaches, in-depth proteomics including analyses of posttranslational modifications and processing, comparative proteomics of plastid ribosomes isolated from the cells grown under different conditions, and those from different taxa are still to be carried out. Establishment of isolation methods for pure plastid ribosomes from a wider range of species would be beneficial to study the relationship between structure, function, and evolution of plastid ribosomes. Here I describe methodologies and provide example protocols for extraction and isolation of plastid ribosomes from a unicellular green alga (Chlamydomonas reinhardtii), a land plant (Arabidopsis thaliana), and a marine red macroalga (Pyropia yezoensis).