IMMUNOGLOBULIN-G INDEPENDENT ACTIVATION OF THE CLASSICAL COMPLEMENT PATHWAY BY MONOSODIUM URATE CRYSTALS
IMMUNOGLOBULIN-G INDEPENDENT ACTIVATION OF THE CLASSICAL COMPLEMENT PATHWAY BY MONOSODIUM URATE CRYSTALS
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DOI：
10.1172/jci109360
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发表时间：
1979-01-01
影响因子：
15.9
通讯作者：
COOPER, NR
中科院分区：
文献类型：
--
作者：
GICLAS, PC;GINSBERG, MH;COOPER, NR
10 mg尿酸钠晶体使1 ml人血清在37℃下加热30 min后总溶血补体CH50降低57%。C. C1、C4和C3耗竭分别为52%、68%和46%，是典型的经典通路激活。当尿酸盐晶体与分离的前体C1孵育时，C1结合和激活发生，并且需要完整的大分子C1qrs。在F（ab”）2抗体阻断聚集性免疫球蛋白对C1的激活的条件下，F（ab”）2抗fc不降低尿酸盐晶体对分离C1的激活。[尿酸钠晶体激活C可能有助于急性痛风的发展]。
Ten mg of monosodium urate crystals reduced the CH50 [total hemolytic complement] of 1 ml of human serum by 57% after 30 min at 37.degree. C. C1, C4 and C3 depletion of 52, 68 and 46% were typical of classical pathway activation. C1 binding and activation occurred when urate crystals were incubated with isolated precursor C1, and required the intact macromolecule, C1qrs. Activation of isolated C1 by urate crystals was not diminished by F(ab'')2 anti-Fc under conditions in which C1 activation by aggregated immunoglobulinG was blocked by the F(ab'')2 antibody. [Activation of C by monosodium urate crystals may contribute to the development of acute gout].