The Expression of Macrophage Migration Inhibitory Factor Is Correlated with Receptor Activator of Nuclear Factor Kappa B Ligand in Induced Rat Periapical Lesions
The Expression of Macrophage Migration Inhibitory Factor Is Correlated with Receptor Activator of Nuclear Factor Kappa B Ligand in Induced Rat Periapical Lesions
复制标题
大鼠根尖周病变中巨噬细胞迁移抑制因子的表达与核因子κB配体受体激活剂相关
DOI：
10.1016/j.joen.2013.03.001
复制
发表时间：
2013-08-01
影响因子：
4.2
通讯作者：
Peng, Bin
中科院分区：
文献类型：
--
作者：
Liu, Lingshuang;Peng, Bin
Introduction: Macrophage migration inhibitory factor (MIF) has been defined as a key cytokine in regulation of innate and adaptive immunity. The purpose of this study was to investigate the immunohistochemical localization of MIF and its relationship with receptor activator of nuclear factor kappa B ligand (RANKL) protein during the development of periapical lesions in rats. Methods: Apical periodontitis was induced in Wistar rats by occlusal pulp exposure in mandibular first molar teeth. The animals were randomly killed at 0, 7, 14, 21, 28, and 35 days after pulp exposure. The jaws that contained the first molar were obtained and were prepared for histologic analysis, enzyme histochemistry, immunohistochemistry, and double immunofluorescence staining. Results: From day 0 to day 35, the areas of periapical bone loss increased and seemed to be stabilized on day 35. A few MIF-positive and RANKL-positive cells and osteoclasts could be observed on day 7, and all climaxed on day 14. From day 21 to day 35, the expression of MIF and RANKL protein decreased, and fewer osteoclasts could be observed. Conclusions: These findings showed that MIF might be associated with the differentiation of osteoclasts in the periapical lesions. MIF contributes to the pathogenesis of the periapical lesions through the induction of RANKL protein.
