A MOUSE HOMOLOG OF DUNCE, A GENE IMPORTANT FOR LEARNING AND MEMORY IN DROSOPHILA, IS PREFERENTIALLY EXPRESSED IN OLFACTORY RECEPTOR NEURONS
A MOUSE HOMOLOG OF DUNCE, A GENE IMPORTANT FOR LEARNING AND MEMORY IN DROSOPHILA, IS PREFERENTIALLY EXPRESSED IN OLFACTORY RECEPTOR NEURONS
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DOI：
10.1002/neu.480280109
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发表时间：
1995-09-01
期刊：
影响因子：
--
通讯作者：
DAVIS, RL
中科院分区：
文献类型：
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作者：
CHERRY, JA;DAVIS, RL
果蝇的dnc （dunce）基因编码环磷酸腺苷特异性磷酸二酯酶（PDE），该位点突变的果蝇表现出严重的学习和记忆缺陷。我们已经开始分析哺乳动物中doe同源物在小鼠中的神经分布，令人惊讶的是，使用四种哺乳动物dnc同源物之一（mPDE2）特异性探针的原位杂交和northern印迹显示嗅觉神经上皮中有高水平表达。抗mPDE2抗体证实，这种PDE蛋白在嗅觉受体神经元的轴突和树突中丰富，但在嗅觉信号转导的初始事件发生的纤毛中明显缺失，在整个大脑和睾丸中也检测到低水平的mPDE2，这些发现表明mPDE2在哺乳动物嗅觉中起重要的调节作用。(C) 1995 John Wiley & Sons, Inc.；
The dunce (dnc) gene in Drosophila codes for a cyclic adenosine monophosphate-specific phosphodiesterase (PDE), Flies with a mutation at this locus exhibit severe deficits in learning and memory, We have begun to analyze the neural distribution of mammalian homologs of doe in the mouse, Surprisingly, in situ hybridization and northern blotting using a probe specific for one of the four mammalian dnc homologs (mPDE2) reveals high levels of expression in the olfactory neuroepithelium. Anti-mPDE2 antibody confirms that this PDE protein is abundant in the axons and dendrites of the olfactory receptor neurons but is conspicuously absent from the cilia, where the initial events in olfactory signal transduction occur, Lower levels of mPDE2 were also detected throughout the brain and in the testis, These findings suggest an important modulatory role for mPDE2 in mammalian olfaction. (C) 1995 John Wiley & Sons, Inc.