Cloning and expression analysis of GmGAL1, SOC1 homolog gene in soybean
Cloning and expression analysis of GmGAL1, SOC1 homolog gene in soybean
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DOI：
10.1007/s11033-012-1524-0
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发表时间：
2012-02
影响因子：
2.8
通讯作者：
X. Zhong;Xiaohua Dai;Jiaohui Xv;Hanying Wu;B. Liu;Hongyu Li
中科院分区：
文献类型：
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作者：
X. Zhong;Xiaohua Dai;Jiaohui Xv;Hanying Wu;B. Liu;Hongyu Li
MADS盒基因AGL20/SOC1是拟南芥开花途径的主要整合子，其结构和功能在许多植物物种中高度保守。从大豆品种Kennong18中克隆了大豆MADS盒基因GmGAL1(Glycine maxAGAMOUSSLike1)作为AGL20/SOC1的同源物，并在转基因拟南芥中研究了其功能。序列比对表明与GmGAL1最接近的同源基因是拟南芥中的AGL20/SOC1和豇豆中的VuSOC1。我们通过实时定量PCR检测了GmGAL1的表达水平，结果表明GmGAL1受到昼夜节律机制的调节，其表达以24小时为周期振荡。 GmGAL1的表达水平在不同组织/器官和发育阶段存在波动。考虑到其在整个生命周期的不同组织中都可以检测到其表达，并且其蛋白定位于拟南芥原生质体的细胞质中，我们推测GmGAL1可能是大豆发育背景下的多功能基因。拟南芥中GmGAL1的异位表达增强了长日照条件下的开花并部分挽救了晚花型。
A MADS box geneAGL20/SOC1is a main integrator inArabidopsisflowering pathway whose structure and function are highly conserved in many plant species. A soybean MADS box geneGmGAL1(Glycine maxAGAMOUSLike1) as a homolog ofAGL20/SOC1, was cloned from soybean cultivar Kennong18 and its function was investigated in transgenicArabidopsislines. Sequence comparisons showed that the closest homolog gene toGmGAL1isAGL20/SOC1inArabidopsisandVuSOC1inVigna unguiculata. We investigated the expression level ofGmGAL1using quantitative real-time PCR, and the result showed thatGmGAL1was regulated by a circadian mechanism and its expression oscillated at a cycle of 24 h. The expression level ofGmGAL1was fluctuated in diverse tissues/organs and developmental stages. Considering its expression can be detected in different tissues throughout the life cycle and its protein localized in cytoplasm inArabidopsisprotoplasm, we proposed thatGmGAL1may be a multifunctional gene in the context of the soybean development. Ectopic expression ofGmGAL1inArabidopsisenhanced flowering under long-day condition and partially rescuedsoc1late flowering type.