Effects of boron starvation on boron compartmentation, and possibly hormone-mediated elongation growth and apical dominance of pea (Pisum sativum) plants
Effects of boron starvation on boron compartmentation, and possibly hormone-mediated elongation growth and apical dominance of pea (Pisum sativum) plants
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DOI：
10.1034/j.1399-3054.2001.1110212.x
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发表时间：
2001-02-01
影响因子：
6.4
通讯作者：
Bangerth, F
中科院分区：
文献类型：
--
作者：
Li, CJ;Pfeffer, H;Bangerth, F
进行了两项实验，以研究在受控生长室条件下缺硼 (B) 对 7 日龄豌豆植株的影响，持续 6 或 9 天。在整个饥饿期间跟踪植物的生长和顶端优势 (AD) 及其 B 浓度和区室划分。此外，还测量了茎尖中的生长素（吲哚乙酸，IAA）浓度及其极性运输，以及茎尖和根中的细胞分裂素（CK）浓度。结果表明，在6天的缺硼期间，根部不溶于水的残留物中硼的浓度非常稳定，不易降低。相反，细胞液部分中的硼浓度对外部硼供应非常敏感。将植物从 B 充足溶液转移至 B 缺乏溶液 12 小时后，根细胞液中的 B 浓度下降至对照植物浓度的一半。此外，在缺乏维生素 B 的处理开始后，新的地上植物部分中的维生素 B 浓度极低。施加 B 缺乏后约 4 天即可观察到伸长增长的下降。这先于侧芽的第一次可测量的生长（从 AD 释放）。在这些形态变化发生之前，CK浓度显着下降，伴随着茎尖IAA输出的急剧减少，茎尖和根部IAA浓度的下降以及极性IAA运输能力的降低。这些变化被讨论为观察到的伸长率增长和 AD 减少的可能原因。这些激素变化本身可能是共质 B 浓度降低的结果，这反过来又可能导致顶端 CK 浓度降低。一系列可能存在因果关系的事件被建议解释 B 缺乏对豌豆植物生长和 AD 的影响。
Two experiments were carried out to study the effects of boron (B) deficiency on 7-day-old pea plants for 6 or 9 days under controlled growth chamber conditions. Growth and apical dominance (AD) of the plants and their B concentration and compartmentation were followed throughout the starvation period. Additionally, auxin (indoleacetic acid, IAA) concentration in the shoot apex and polar transport from it were measured along with the cytokinin (CK) concentration in the shoot apex and the roots. The results demonstrate that during a 6-day B-deficiency period, B concentration in the water-insoluble residue of the roots was very stable and could not easily be reduced. In contrast, B concentration in the cell sap fraction was very sensitive to external B supply. Twelve hours after transferring the plants from B-sufficient to B-deficient solutions, the B concentration in root cell sap declined to half the concentration of the control plants. In addition, B concentration in the new aerial plant parts, which developed after the onset of the B-deficiency treatment, was extremely low. A decline in elongation growth could be observed as soon as about 4 days after the imposition of B deficiency. This preceded the first measurable growth of lateral buds (release from AD). Before the onset of these morphological changes, there was a considerable decline in CK concentration, accompanied by a dramatic decrease in IAA export out of the shoot apex, a decline in IAA concentration in the shoot apex and the roots and a reduced capacity for polar IAA-transport. These changes are discussed as possible reasons for the observed reduction in elongation growth and AD. These hormonal changes themselves are possibly the result of the decreased symplasmic B concentration, which in turn may be responsible for the reduced concentration in apical CKs, A sequence of events, which may be causally related, is suggested to explain the effects of B deficiency on the growth and AD of pea plants.