Plasma Lithography Surface Patterning for Creation of Cell Networks
Plasma Lithography Surface Patterning for Creation of Cell Networks
复制标题
DOI：
10.3791/3115
复制
发表时间：
2011-06-01
影响因子：
1.2
通讯作者：
Wong, Pak Kin
中科院分区：
文献类型：
--
作者：
Junkin, Michael;Leung, Siu Ling;Wong, Pak Kin
对于细胞微环境的系统操作，通常需要微纳米级的分辨率来破译各种细胞和分子现象。为了满足这一要求，我们开发了一种等离子光刻技术，通过创建特征尺寸从100纳米到毫米的图案表面来操纵细胞微环境。这项技术的目标是能够以可控的方式研究单个细胞和细胞群的行为及其相互作用。这种等离子体光刻方法是通过屏蔽低温等离子体与物理模具的接触，对基材表面化学进行选择性修饰。这种选择性屏蔽留下了一种化学模式，可以指导细胞的附着和运动。这种模式或表面模板可以用来创建细胞网络，其结构可以模仿自然界中发现的结构，并为实验研究创造一个可控的环境。该技术非常适合研究生物现象，因为它以生物相容的方式在透明聚合物基底上产生稳定的表面图案。表面图案可以持续数周到数月，因此可以长时间指导与细胞的相互作用，从而促进长期细胞过程的研究，如分化和适应。对表面的修饰主要是化学性质的，因此不会引入地形或物理干扰来解释结果。它也不涉及任何苛刻或有毒的物质，以实现图案和兼容的组织培养。此外，它可以应用于修饰各种类型的聚合物底物，由于能够调节其性质，因此在生物应用中是理想的并被广泛应用。可实现的分辨率也是有益的，因为对迁移、粘附或结合等特定过程的隔离允许在单个到多细胞水平上进行离散、清晰的观察。该方法已被用于形成不同细胞类型的不同网络，用于涉及迁移、信号、组织形成以及网络中神经元的行为和相互作用的研究。
Systematic manipulation of a cell microenvironment with micro- and nanoscale resolution is often required for deciphering various cellular and molecular phenomena. To address this requirement, we have developed a plasma lithography technique to manipulate the cellular microenvironment by creating a patterned surface with feature sizes ranging from 100 nm to millimeters. The goal of this technique is to be able to study, in a controlled way, the behaviors of individual cells as well as groups of cells and their interactions.This plasma lithography method is based on selective modification of the surface chemistry on a substrate by means of shielding the contact of low-temperature plasma with a physical mold. This selective shielding leaves a chemical pattern which can guide cell attachment and movement. This pattern, or surface template, can then be used to create networks of cells whose structure can mimic that found in nature and produces a controllable environment for experimental investigations. The technique is well suited to studying biological phenomenon as it produces stable surface patterns on transparent polymeric substrates in a biocompatible manner. The surface patterns last for weeks to months and can thus guide interaction with cells for long time periods which facilitates the study of long-term cellular processes, such as differentiation and adaption. The modification to the surface is primarily chemical in nature and thus does not introduce topographical or physical interference for interpretation of results. It also does not involve any harsh or toxic substances to achieve patterning and is compatible for tissue culture. Furthermore, it can be applied to modify various types of polymeric substrates, which due to the ability to tune their properties are ideal for and are widely used in biological applications. The resolution achievable is also beneficial, as isolation of specific processes such as migration, adhesion, or binding allows for discrete, clear observations at the single to multicell level.This method has been employed to form diverse networks of different cell types for investigations involving migration, signaling, tissue formation, and the behavior and interactions of neurons arraigned in a network.