Selective differentiation of mammalian bone marrow stromal cells cultured on three-dimensional polymer foams
Selective differentiation of mammalian bone marrow stromal cells cultured on three-dimensional polymer foams
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DOI：
10.1002/1097-4636(200105)55:2
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发表时间：
2001-05-01
期刊：
影响因子：
--
通讯作者：
Freed, LE
中科院分区：
文献类型：
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作者：
Martin, I;Shastri, VP;Freed, LE
骨髓基质细胞（BMSC）是多能祖细胞，可以响应环境信号再生不同的骨骼组织。在这项研究中，我们使用高度多孔，结构稳定的三维聚合物泡沫结合特定的调节分子，选择性地分化成软骨或骨样组织的哺乳动物BMSC。将牛BMSC单层扩增，并在由聚（乳酸-共-乙醇酸）和聚（乙二醇）制成的5 mm直径、2 mm厚的泡沫上培养。构建体在长达4周的培养中保持其原始大小和形状，并支持BMSC生长和细胞外基质（ECM）的产生。通过适当使用软骨形成（地塞米松，胰岛素，转化生长因子-β 1）或成骨（地塞米松，β-甘油磷酸盐）培养基补充剂，我们可以控制所产生的ECM是软骨性的（含有II型胶原蛋白和硫酸化糖胺聚糖）还是骨样的（含有骨钙素，骨粘连蛋白和矿化灶）。培养4周后，软骨和骨样ECM均匀分布在整个构建体体积中，分别占总可用面积的34.2 +/- 9.3%和12.6 +/- 3.2%。BMSC在聚乳酸-羟基乙酸共聚物/聚乙二醇泡沫上的培养为研究间充质组织的体外发育提供了一个三维模型系统，并在工程化自体骨组织修复中具有潜在的应用前景。(C)John Wiley & Sons，Inc.
Bone marrow stromal cells (BMSC) are pluripotent progenitor cells that can regenerate different skeletal tissues in response to environmental signals. In this study, we used highly porous, structurally stable three-dimensional polymer foams in conjunction with specific regulatory molecules to selectively differentiate mammalian BMSC into either cartilaginous or bone-like tissues. Bovine BMSC were expanded in monolayers and cultured on 5-mm-diameter, 2-mm-thick foams made of poly(lactic-co-glycolic acid) and poly(ethylene glycol). Constructs maintained their original size and shape for up to 4 weeks of culture and supported BMSC growth and production of extracellular matrix (ECM). By proper use of chondrogenic (dexamethasone, insulin, transforming growth factor-pi) or osteogenic (dexamethasone, p-glycerophosphate) medium supplements, we could control whether the generated ECM was cartilaginous (containing collagen type II and sulfated glycosaminoglycans) or bone-like (containing osteocalcin, osteonectin, and mineralized foci). After 4 weeks of cultivation, cartilaginous and bone-like ECM were uniformly distributed throughout the construct volume and respectively represented 34.2 +/- 9.3% and 12.6 +/- 3.2% of the total available area. BMSC culture on poly(lactic-co-glycolic acid)/ poly(ethylene glycol) foams provides a three-dimensional model system to study the development of mesenchymal tissues in vitro and has potential applications in engineering autologous grafts for skeletal tissue repair. (C) 2001 John Wiley & Sons, Inc.