A novel control process of cyanobacterial bloom using cyanobacteriolytic bacteria immobilized in floating biodegradable plastic carriers
A novel control process of cyanobacterial bloom using cyanobacteriolytic bacteria immobilized in floating biodegradable plastic carriers
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DOI：
10.1080/09593330309385703
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发表时间：
2003-12
影响因子：
2.8
通讯作者：
Nobuyuki Nakamura;Kazunori Nakano;Norio Sugiura;Masatoshi Matsumura
中科院分区：
文献类型：
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作者：
Nobuyuki Nakamura;Kazunori Nakano;Norio Sugiura;Masatoshi Matsumura
摘要为实现对天然漂浮蓝藻水华的现场有效控制，提出了一种利用漂浮载体固定化溶蓝细菌的工艺。研究了蓝藻溶解物质和蜡状芽孢杆菌N-14细胞表现出蓝藻溶解活性所需的临界浓度。结果表明，细胞生长需要产生足够多的蓝藻溶解物质以展示其活性，也需要能够使高浓度的蓝藻溶解物质与蓝藻良好接触的条件。采用淀粉制成的漂浮生物降解塑料作为载体材料，使固定化蓝藻与漂浮蓝藻保持紧密接触。漂浮淀粉载体在4d内可去除99%的漂浮蓝藻，蜡状芽孢杆菌N-14在淀粉和部分氨基酸存在下能产生溶蓝藻物质，其溶藻活性归因于淀粉载体提供的碳源和裂解蓝藻洗脱出的氨基酸。因此，从固定化载体和营养源两个角度证实了漂浮淀粉载体对蓝藻的促进作用。论证了应用固定化有用微生物的漂浮载体对有害漂浮目标进行强化处理的新概念。
Abstract A process using a floating carrier for immobilization of cyanobacteriolytic bacteria, B.cereus N‐14, was proposed to realize an effective in situ control of natural floating cyanobacterial blooms. The critical concentrations of the cyanobacteriolytic substance and B.cereus N‐14 cells required to exhibit cyanobacteriolytic activity were investigated. The results indicated the necessity of cell growth to produce sufficiently high amounts of the cyanobacteriolytic substance to exhibit its activity and also for conditions enabling good contact between high concentrations of the cyanobacteriolytic substance and cyanobacteria. Floating biodegradable plastics made of starch were applied as a carrier material to maintain close contact between the immobilized cyanobacteriolytic bacteria and floating cyanobacteria. The floating starch‐carriers could eliminate 99% of floating cyanobacteria in 4 d. Since B.cereus N‐14 could produce the cyanobacteriolytic substance under the presence of starch and some amino acids, the cyanobacteriolytic activity could be attributed to carbon source fed from starch carrier and amino acids eluted from lysed cyanobacteria. Therefore, the effect of using a floating starch‐carrier was confirmed from both view points as a carrier for immobilization and a nutrient source to stimulate cyanobacteriolytic activity. The new concept to apply a floating carrier immobilizing useful microorganisms for intensive treatment of a nuisance floating target was demonstrated.