Proteome changes on water-holding capacity of yak longissimus lumborum during postmortem aging
Proteome changes on water-holding capacity of yak longissimus lumborum during postmortem aging
复制标题
宰后老化过程中牦牛腰最长肌持水能力的蛋白质组变化
DOI：
10.1016/j.meatsci.2016.07.010
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发表时间：
2016
期刊：
影响因子：
7.1
通讯作者：
Shi Hongmei
中科院分区：
文献类型：
--
作者：
Zuo Huixin;Han Ling;Yu Qunli;Niu Kelan;Zhao Suonan;Shi Hongmei
为了研究腰长肌死后老化过程中持水能力（WHC）的差异表达蛋白，根据滴漏损失将样品分为高、低滴漏损失组。55种蛋白在死后衰老的第0、1和7天差异丰富，并通过MALDI TOF/TOF鉴定。鉴定的蛋白质可分为四大类：代谢酶、细胞结构蛋白、应激相关蛋白和转运蛋白。肌球蛋白轻链、热休克蛋白27和三磷酸异构体酶在两组间存在较大差异，可能具有作为WHC预测的生物学标志物的潜力。此外，生物信息学分析表明，鉴定的蛋白质与碳代谢、糖酵解、氨基酸生物合成和丙酮酸代谢有关。所鉴定的蛋白质的功能有助于对WHC背后的过程有更详细的分子视图，并且是未来研究的宝贵资源。
To study differentially expressed proteins on water-holding capacity (WHC) during postmortem aging oflongissimuslumborummuscle, samples were classified according to drip loss into high and low drip loss groups. Fifty-five proteins were differentially abundant at days 0, 1 and 7 during postmortem aging and identified by MALDI TOF/TOF. The identified proteins can be divided into four main categories: metabolic enzymes, cell structural proteins, stress related proteins and transport proteins. Myosin light chain, heat shock protein 27 and triosephosphate isomerase showed a major difference between the two groups and may have the potential to be biological markers for WHC prediction. Furthermore, bioinformatics analysis revealed that the identified proteins were related to carbon metabolism, glycolysis and biosynthesis of amino acids and pyruvate metabolism. The functions of the identified proteins contribute to a more detailed molecular view of the processes behind WHC and are a valuable resource for future investigations.