Bone marrow aspiration
Bone marrow aspiration
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DOI：
10.1136/jcp.54.9.657
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发表时间：
2001-09-01
影响因子：
3.4
通讯作者：
Bain, BJ
中科院分区：
文献类型：
--
作者：
Bain, BJ
进行骨髓活检主要是为了进行细胞学评估，但也是为了免疫表型、细胞遗传学、分子遗传学和其他专门研究。通常，环钻活组织检查是同一程序的一部分。骨髓抽吸应由训练有素的人员进行，他们知道该手术的适应症、禁忌症和危险。他们应该遵循标准的操作程序。操作员应该对临床和血液学特征进行充分的评估，以确保存在适当的适应症并进行所有相关的测试。为了患者的舒适性和安全性，髂骨后缘通常是首选的抽吸部位。应制作抽吸骨髓的薄膜，并在适当的情况下，制作粉碎颗粒的薄膜并贴上标签。一旦完全干燥，胶片应该固定并染色。至少需要罗曼诺斯基染色和珀尔斯染色。应该贴上盖子。骨髓片应该以系统的方式进行评估和报告，这样就不会忽视任何重要的东西，使用低倍率，然后是中等倍数，然后是高倍率的物镜。应执行差异计数。应根据临床和血液学特征对结果进行解释。报告应签署或电脑授权，使用安全密码，并及时发布。
Bone marrow aspiration biopsies are carried out principally to permit cytological assessment but also for immunophenotypic, cytogenetic, molecular genetic, and other specialised investigations. Often, a trephine biopsy is carried out as part of the same procedure. Bone marrow aspirations should be carried out by trained individuals who are aware of the indications, contraindications, and hazards of the procedure. They should follow a standard operating procedure. The operator should have made an adequate assessment of clinical and haematological features to ensure both that appropriate indications exist and that all relevant tests are performed. For the patient's comfort and safety, the posterior iliac crest is generally the preferred site of aspiration. Films of aspirated marrow and, when appropriate, films of crushed particles should be made and labelled. Once thoroughly dry, films should be fixed and stained. As a minimum, a Romanowsky stain and a Perls' stain are required. A cover slip should be applied. The bone marrow films should be assessed and reported in a systematic manner so that nothing of importance is overlooked, using a low power, then intermediate, then high power objective. A differential count should be performed. An interpretation of the findings, in the light of the clinical and haematological features, should be given. The report should be signed or computer authorised, using a secure password, and issued in a timely manner.