Glutamatergic and GABAergic neurons in the vLGN mediate the nociceptive effects of green and red light on neuropathic pain
Glutamatergic and GABAergic neurons in the vLGN mediate the nociceptive effects of green and red light on neuropathic pain
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DOI：
10.1016/j.nbd.2023.106164
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发表时间：
2023-05-25
影响因子：
6.1
通讯作者：
Chen，Zhong
中科院分区：
文献类型：
--
作者：
Wu，Xue-Qing;Tan，Bei;Chen，Zhong
光疗是一种新兴的非药物治疗抑郁症，昼夜节律中断，神经退行性疾病，以及疼痛的条件，包括偏头痛和纤维肌痛。然而，光疗诱导的抗伤害感受的机制尚不清楚。在这里，使用纤维光度计记录的人口水平的神经活动结合化学遗传学，我们发现，光疗elephant antinociception通过调节位于视觉系统中的腹外侧膝状体（vLGN）。具体而言，绿色和红色光都引起vLGN中c-fos的增加，红色光增加更多。在vLGN中，绿色光引起谷氨酸能神经元的大量增加，而红光引起GABA能神经元的大量增加。绿色光预处理可增加PSL小鼠vLGN内多巴胺能神经元对伤害性刺激的敏感性。绿色光通过激活vLGN中的GABA能神经元产生抗伤害感受，红光通过激活vLGN中的GABA能神经元促进伤害感受。总之，这些结果表明，不同颜色的光通过调节vLGN中的谷氨酸能和GABA能亚群来发挥不同的疼痛调节作用。这可能为神经病理性疼痛的精确临床治疗提供潜在的新的治疗策略和新的治疗靶点。
Phototherapy is an emerging non-pharmacological treatment for depression, circadian rhythm disruptions, and neurodegeneration, as well as pain conditions including migraine and fibromyalgia. However, the mechanism of phototherapy-induced antinociception is not well understood. Here, using fiber photometry recordings of population-level neural activity combined with chemogenetics, we found that phototherapy elicits antinociception via regulation of the ventral lateral geniculate body (vLGN) located in the visual system. Specifically, both green and red lights caused an increase of c-fos in vLGN, with red light increased more. In vLGN, green light causes a large increase in glutamatergic neurons, whereas red light causes a large increase in GABAergic neurons. Green light preconditioning increases the sensitivity of glutamatergic neurons to noxious stimuli in vLGN of PSL mice. Green light produces antinociception by activating glutamatergic neurons in vLGN, and red light promotes nociception by activating GABAergic neurons in vLGN. Together, these results demonstrate that different colors of light exert different pain modulation effects by regulating glutamatergic and GABAergic subpopulations in the vLGN. This may provide potential new therapeutic strategies and new therapeutic targets for the precise clinical treatment of neuropathic pain.