Cell infiltrative hydrogel fibrous scaffolds for accelerated wound healing.
Cell infiltrative hydrogel fibrous scaffolds for accelerated wound healing.
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DOI：
10.1016/j.actbio.2016.11.017
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发表时间：
2017-02
影响因子：
9.7
通讯作者：
Khademhosseini A
中科院分区：
文献类型：
--
作者：
Zhao X;Sun X;Yildirimer L;Lang Q;Lin ZYW;Zheng R;Zhang Y;Cui W;Annabi N;Khademhosseini A
开发具有可调物理性能和生物相容性的天然蛋白质纤维支架是构建三维、完全细胞化的伤口愈合支架的迫切需要。在这里，我们展示了一种简单而有效的技术，利用基于甲基丙烯酰化明胶(GelMA)的光交联水凝胶构建电纺3D纤维支架以加速伤口愈合。我们发现，光交联水凝胶的物理性能，包括保水性、刚性、强度、弹性和降解性，可以通过改变光照时间来定制。我们进一步观察到，优化的水凝胶纤维支架柔软、有弹性，能够支持细胞黏附、增殖和迁移到整个支架中，促进两周内皮肤组织的再生和形成。纤维状GelMA支架的这种可调特性使其有别于其他报道的用于重建伤口缺损的基质，包括戊二醛交联明胶或聚(乳酸-乙醇酸)(PLGA)，后者的物理和化学性质很难改变，以允许细胞渗透到3D支架中进行组织再生。我们预计，完全细胞化的能力将使工程GelMA水凝胶纤维支架适合作为皮肤替代品或伤口敷料的广泛应用。
Development of natural protein-based fibrous scaffolds with tunable physical properties and biocompatibility is highly desirable to construct a three-dimensional (3D), fully cellularized scaffold for wound healing. Herein, we demonstrated a simple and effective technique to construct electrospun 3D fibrous scaffolds for accelerated wound healing using a photocrosslinkable hydrogel based on methacryloylated gelatin (GelMA). We found that the physical properties of the photocrosslinkable hydrogel including water retention, stiffness, strength, elasticity and degradation can be tailored by changing the light exposure time. We further observed that the optimized hydrogel fibrous scaffolds which were soft and elastic could support cell adhesion, proliferation and migration into the whole scaffolds, facilitating regeneration and formation of cutaneous tissues within two weeks. Such tunable characteristics of the fibrous GelMA scaffolds distinguished them from other reported substrates developed for reconstruction of wound defects including glutaraldehyde-crosslinked gelatin or poly (lactic-co-glycolic acid) (PLGA), whose physical and chemical properties were difficult to modify to allow cell infiltration into the 3D scaffolds for tissue regeneration. We anticipate that the ability to become fully cellularized will make the engineered GelMA hydrogel fibrous scaffolds suitable for widespread applications as skin substitutes or wound dressings.