Comparison of blood sirolimus, tacrolimus and everolimus concentrations measured by LC-MS/MS, HPLC-UV and immunoassay methods
Comparison of blood sirolimus, tacrolimus and everolimus concentrations measured by LC-MS/MS, HPLC-UV and immunoassay methods
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DOI：
10.1016/j.clinbiochem.2010.10.005
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发表时间：
2011-02-01
影响因子：
2.8
通讯作者：
Morris, Raymond G.
中科院分区：
文献类型：
--
作者：
Sallustio, Benedetta C.;Noll, Benjamin D.;Morris, Raymond G.
目的：建立同时测定全血中他克莫司、西罗莫司和依维莫司含量的LC-MS/MS方法，并与HPLC-UV和免疫分析法进行比较。设计与方法：血液（0.1 mL）采用C18柱（45℃）固相萃取和色谱分离，流动相为甲醇/40 mM醋酸铵/冰醋酸（83/17/0.1），浓度为200 μ L/inin，电喷雾正电离，多重反应监测。结果：在合并患者样本中测量的FPIA依维莫司浓度与无药全血中检测的依维莫司浓度相比，日内和日间的不精确和不准确偏差为30%。结论：LC-MS/MS与HPLC和免疫分析法相比，具有显著的准确性和精密度优势。Seradyn FPIA免疫分析法测量的依维莫司浓度差异需要进一步研究。(C) 2010加拿大临床化学学会。Elsevier Inc.出版。版权所有。
Objectives: An LC-MS/MS method was developed for simultaneous quantitation of tacrolimus, sirolimus and everolimus in whole blood, and compared to HPLC-UV and immunoassay methods.Design and methods: Blood (0.1 mL) was analysed following solid-phase extraction and chromatographic resolution using a C18 column (45 degrees C) and mobile phase of methanol/40 mM ammonium acetate/glacial acetic acid (83/17/0.1) at 200 mu L/inin, with positive electrospray ionisation and multiple reaction monitoring.Results: Intra- and inter-day imprecision and inaccuracy were 30% bias in FPIA everolimus concentrations measured in pooled patient samples versus spiked drug-free whole blood.Conclusions: LC-MS/MS provides significant accuracy and precision advantages compared to HPLC and immunoassays. Discrepancies in everolimus concentrations measured by the Seradyn FPIA immunoassay require further investigation. (C) 2010 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.