Spermatogonial SOHLH1 nucleocytoplasmic shuttling associates with initiation of spermatogenesis in the rhesus monkey (Macaca mulatta)
Spermatogonial SOHLH1 nucleocytoplasmic shuttling associates with initiation of spermatogenesis in the rhesus monkey (Macaca mulatta)
复制标题
精原细胞 SOHLH1 核细胞质穿梭与恒河猴（Macaca mulatta）精子发生的启动相关
DOI：
10.1093/molehr/gat093
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发表时间：
2014
期刊：
影响因子：
4
通讯作者：
*Plant TM.
中科院分区：
文献类型：
--
作者：
Ramaswamy S; Razack BS; Roslund RM; Suzuki H; Marshall GR; Rajkovic A; *Plant TM.
由于精子发生和胚胎发育特异性的basichelix-loop-helix 1（SOHLH 1）转录因子已被证明是小鼠精原细胞分化所必需的，我们检测了这种蛋白在青春期恒河猴（Macaca mulatta）睾丸中的免疫表达，青春期是高等灵长类动物精原细胞分化开始的发育阶段。免疫阳性SOHLH 1细胞仅见于生精索和曲细精管的基底膜上。在青春期之前，基本上100%的SOHLH 1阳性精原细胞共表达胶质细胞系源性神经营养因子家族受体α 1（GFRα1），这是未分化精原细胞的标志物，并且>80%的免疫阳性SOHLH 1细胞仅表现出该转录因子的胞质染色。在青春期前动物的睾丸中，在<10%的精原细胞中发现了仅有核的SOHLH 1。青春期与仅核染色的免疫阳性SOHLH 1细胞百分比的急剧和进行性增加相关，这与（i）共表达GFRα1的SOHLH 1阳性生殖细胞比例（约100-20%）显著减少和（ii）共表达酪氨酸激酶受体（cKIT）的SOHLH 1阳性精原细胞比例显著增加相关。发现表现出核SOHLH 1染色的精原细胞是cKIT阳性的，但并非所有cKIT阳性的精原细胞都表现出核SOHLH 1染色。总之，这些结果表明，在猴子，SOHLH 1的核定位与精原细胞分化密切相关。
As thespermatogenesis- andoogenesis-specific basichelix-loop-helix1(SOHLH1) transcription factor has been shown to be essential for spermatogonial differentiation in mice, we examined the immunoexpression of this protein in the testis of the rhesus monkey (Macaca mulatta) during puberty, the stage of development when spermatogonial differentiation is initiated in higher primates. Immunopositive SOHLH1 cells were observed only on the basement membrane of the seminiferous cords and tubules. Prior to puberty, essentially 100% of SOHLH1-positive spermatogonia co-expressed the glial cell line-derived neurotrophic factor family receptor alpha 1 (GFRα1), a marker for undifferentiated spermatogonia, and >80% of the immunopositive SOHLH1 cells exhibited only cytoplasmic staining of this transcription factor. Nuclear-only SOHLH1 was found in <10% of spermatogonia in testes from pre-pubertal animals. Puberty was associated with a dramatic and progressive increase in the percentage of immunopositive SOHLH1 cells with nuclear-only staining, and this was associated with (i) a marked reduction in the fraction (∼100–20%) of SOHLH1-positive germ cells co-expressing GFRα1 and (ii) a significant increase in the proportion of SOHLH1-positive spermatogonia that co-expressed the tyrosine kinase receptor (cKIT). Spermatogonia exhibiting nuclear SOHLH1 staining were found to be cKIT positive, but not all cKIT-positive spermatogonia exhibited nuclear SOHLH1 staining. Taken together, these results suggest that, in the monkey, nuclear location of SOHLH1 is closely associated with spermatogonial differentiation.