The small subunit of the splicing factor U2AF is conserved in fission yeast.
The small subunit of the splicing factor U2AF is conserved in fission yeast.
复制标题
剪接因子 U2AF 的小亚基在裂殖酵母中是保守的。
DOI：
10.1093/nar/24.10.1849
复制
发表时间：
1996
影响因子：
14.9
通讯作者：
Potashkin,J
中科院分区：
文献类型：
--
作者：
Wentz-Hunter,K;Potashkin,J
人剪接因子U2辅助因子（hsU 2AF）由65和35 kDa的两个相互作用的亚基组成。以前，我们确定了裂殖酵母pombehomolog，spU 2AF 59，人类大亚基。我们利用酵母双杂交系统从裂殖酵母cDNA文库中筛选与spU 2AF 59相互作用的蛋白质，并鉴定了hsU 2AF 35亚基的同源物。粟酒裂殖酵母U2 AF小亚基是一个单拷贝基因，其编码的蛋白质与人类小亚基具有55%的氨基酸同一性和17%的相似性。与人类蛋白质不同，酵母蛋白质缺乏富含精氨酸/丝氨酸的区域。spU 2AF小亚基的预测分子量为23 kDa。spU 2AF 59与spU 2AF 23相互作用的区域与人小亚基和大亚基相互作用的区域相似。
The human splicing factor U2 auxiliary factor (hsU2AF) is comprised of two interacting subunits of 65 and 35 kDa. Previously we identified theSchizosaccharomyces pombehomolog, spU2AF59, of the human large subunit. We have screened a fission yeast cDNA library in search of proteins that interact with spU2AF59using the yeast two-hybrid system and have identified a homolog of the hsU2AF35subunit. TheS.pombeU2AF small subunit is a single copy gene that encodes a protein which shares 55% amino acid identity and 17% similarity with the human small subunit. Unlike the human protein, the yeast protein lacks an arginine/serine-rich region. The predicted molecular mass of the spU2AF small subunit is 23 kDa. The region of spU2AF59that interacts with spU2AF23is similar to the region in which the human small and large subunits interact.
DOI：
10.1101/sqb.1986.051.01.032
发表时间：
1986-01-01
期刊：
COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY
影响因子：
--
作者：
MULLIS, K;FALOONA, F;ERLICH, H
通讯作者：
ERLICH, H