Isolation and properties of a single-strand 5'----3' exoribonuclease from Ehrlich ascites tumor cell nucleoli.
Isolation and properties of a single-strand 5'----3' exoribonuclease from Ehrlich ascites tumor cell nucleoli.
复制标题
艾利希腹水肿瘤细胞核仁中单链 5----3 核糖核酸酶的分离和特性。
DOI：
10.1021/bi00314a019
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发表时间：
1984
期刊：
影响因子：
2.9
通讯作者：
Eichler,DC
中科院分区：
文献类型：
--
作者：
Lasater,LS;Eichler,DC
材料和方法 材料。未标记和标记的[3H]聚(C)(22.8 mCi/mmol)、[3H]聚(A)(42 mCi/mmol)、[3H]聚(U)(525 mCi/mmol)、[3H]聚(C)-聚(I)(9.8 mCi/mmol)和[3H]聚(U)-聚(A)(19.6 mCi/mmol)来自Miles Chemicals Corp. 胞苷 3', 5'-[5'-32P] 二磷酸 (2000 Ci/mmol) 购自 ICN。[-32](1000-2000 Ci/mmol) 按照 Maxam & Gilbert (1977) 的描述制备。胞苷S'。 S'-二磷酸购自PL Biochemicals。染料结合蛋白测定试剂盒购自Bio-Rad。聚(氮丙啶)浸渍的纤维素薄层色谱板购自Brinkmann.Proteins and Enzymes。细菌碱性磷酸酶和T4多核苷酸激酶购自Bethesda研究实验室。 St核酸酶和T4 RNA连接酶获自P-L Biochemicals。牛血清白蛋白、卵白蛋白和酵母乙醇脱氢酶购自Sigma。人胎盘核糖核酸酶抑制剂按照Blackburn(1979)的方法制备。 RNA。 32P 标记的 f2 RNA (4 X 104* cpm/nmol) 基本上是根据 Glitz (1968) 的 MS2 RNA 程序制备的，同时对此进行了修改
Materials and Methods Materials. Unlabeled and labeled [3H] poly (C)(22.8 mCi/mmol),[3H] poly (A)(42 mCi/mmol),[3H] poly (U)(525 mCi/mmol),[3H] poly (C)-poly (I)(9.8 mCi/mmol), and [3H] poly (U)-poly (A)(19.6 mCi/mmol) were obtained from Miles Chemicals Corp. Cytidine 3', 5'-[5'-32P] diphosphate (2000 Ci/mmol) was purchased from ICN.[-32](1000-2000 Ci/mmol) was prepared as described byMaxam & Gilbert (1977). Cytidine S'. S'-diphosphate was purchased from PL Biochemicals. The dye-binding protein assay kit was obtained from Bio-Rad. Poly (ethylenimine)-impregnated cellulose thin-layer chromatography plates were purchased from Brinkmann.Proteins and Enzymes. Bacterial alkaline phosphatase and T4 polynucleotide kinase were purchased from Bethesda Research Laboratories. St nuclease and T4 RNA ligase were obtained fromP-L Biochemicals. Bovine serum albumin, ovalbumin, andyeast alcohol dehydrogenase were obtained from Sigma. Human placentalribonuclease inhibitor was prepared by the method of Blackburn (1979). RNAs. 32P-Labeled f2 RNA (4 X 104* cpm/nmol) was prepared essentially according to the procedure of Glitz (1968) for MS2 RNA, and at the same time, modifications of this