QUANTITATIVE ASSESSMENT OF ATHEROSCLEROTIC LESIONS IN MICE
QUANTITATIVE ASSESSMENT OF ATHEROSCLEROTIC LESIONS IN MICE
复制标题
DOI：
10.1016/0021-9150(87)90202-4
复制
发表时间：
1987-12-01
期刊：
影响因子：
5.3
通讯作者：
WILLIAMS, RA
中科院分区：
文献类型：
--
作者：
PAIGEN, B;MORROW, A;WILLIAMS, RA
小鼠明确的遗传系统被证明在动脉粥样硬化的实验研究中是有用的。近亲繁殖的小鼠品系在动脉粥样硬化易感性方面存在差异，并且已经鉴定和绘制了载脂蛋白的几种变体。本报告探讨了小鼠病变形成的位置和时间，旨在为定量比较小鼠组提供基础。C57 BL/6 J雌性小鼠在食用含有1.25%胆固醇、15%脂肪和0.5%胆酸的致动脉粥样硬化饮食14周后，在每根肋间动脉、主动脉与心脏的连接处以及覆盖1.1% ± 1.5%的分散区域中出现主动脉病变。0.5（SD）的主动脉表面。在致动脉粥样硬化饮食9个月后，心脏和肋间动脉附近的病变广泛，为8% ± 10%。3例（SD）主动脉其余部分受累，冠状动脉中发现病变。结果表明，对损伤进行评分的一个合适位置是在主动脉窦之外的300 μ m的主动脉区域中。在致动脉粥样硬化饮食14周后，所选区域中每只小鼠的平均损伤数为1.1 ± 1.1。0.3（SD）。结果在10个单独的实验中是可重复的。每只小鼠的病变数量符合泊松分布，表明一个病变的存在不会使小鼠易于获得第二个病变。病变的形成和胆固醇水平并没有随着季节的变化而变化，这一点在超过12个月的9个独立实验中得到了证明。比较了评估病变数量和大小的方法，包括显微镜目镜网格和计算机辅助平面测量法。所得数据提供了定量比较不同品系或小鼠组中病变形成的可重复方法，从而增加了小鼠作为动脉粥样硬化研究实验系统的有用性。
The well-defined genetic systems of the mouse are proving useful in experimental studies of atherosclerosis. Inbred mouse strains differ in atherosclerosis susceptibility, and several variants of apolipoproteins have been identified and mapped. This report explores the location and timing of lesion formation in the mouse in an effort to provide a basis for quantitatively comparing groups of mice. After 14 weeks on an atherogenic diet containing 1.25% cholesterol, 15% fat, and 0.5% cholic acid, C57BL/6J female mice had aortic lesions at each of the intercostal arteries, at the junction of the aorta to the heart, and in scattered areas covering 1.1% .+-. 0.5 (SD) of the aortic surface. After 9 months on the atherogenic diet, those lesions near the heart and intercostal arteries were extensive, 8% .+-. 3 (SD) of the remainder of the aorta was involved in lesions, and lesions were found in the coronary arteries. Results indicated that one suitable location for scoring lesions was in a 300 .mu.m area of the aorta just beyond the aortic sinus. The mean number of lesions/mouse in the selected area after 14 weeks on the atherogenic diet was 1.1 .+-. 0.3 (SD). The results were reproducible over 10 separate experiments. The number of lesions per mouse fit a Poisson distribution indicating that the presence of one lesion did not predispose the mouse to acquiring a second lesion. Lesion formation and cholesterol levels did not vary with the season of the year as demonstrated by 9 separate experiments over more than 12 months. Methods of evaluating the number and size of lesions were compared including sizing with a microscope eyepiece grid and computer-assisted planimetry. The resulting data provide reproducible methods of quantitatively comparing lesion formation in various strains or groups of mice, thereby increasing the usefulness of the mouse as an experimental system for atherosclerosis research.