Detection of respiratory viruses using a multiplex real-time PCR assay in Germany, 2009/10
Detection of respiratory viruses using a multiplex real-time PCR assay in Germany, 2009/10
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DOI：
10.1007/s00705-013-1876-3
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发表时间：
2014-04-01
影响因子：
2.7
通讯作者：
Panning, Marcus
中科院分区：
文献类型：
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作者：
Bierbaum, Sibylle;Forster, Johannes;Panning, Marcus
这项研究的目的是确定呼吸道病毒的流行率，并前瞻性地评估2009/10年流感大流行后不久快速诊断(FTD)呼吸道病原体多重PCR检测的性能。以高灵敏的单链实时荧光聚合酶链式反应检测为参考。用xTAG RVP Fast法对不一致的结果进行进一步分析。从2009年12月到2010年6月，德国前瞻性地收集了369份儿童和成人的呼吸道样本。FTD法的灵敏度为92.2%，特异度为99.5%。FTD试验对人类博卡病毒的特异性最低。单链聚合酶链式反应多重检测33/369例(8.9%)，FTD法检测43/369例(11.7%)。最流行的病毒是呼吸道合胞病毒和人偏肺病毒。只检测到大流行性流感病毒A/H1N1(2009年)，而不是季节性流感病毒。流感病毒以外的病毒占急性呼吸道感染的大部分。FTD分析可以很容易地在普通诊断实验室中实施，并有助于优化患者管理方案。
The aim of this study was to determine the prevalence of respiratory viruses and to prospectively evaluate the performance of the fast-track diagnostics (FTD) respiratory pathogens multiplex PCR assay shortly after the 2009/10 influenza pandemic. Highly sensitive monoplex real-time PCR assays served as references. Discrepant results were further analyzed by the xTAG RVP Fast assay. A total of 369 respiratory samples from children and adults were collected prospectively in Germany from December 2009 until June 2010. The sensitivity and specificity of the FTD assay after resolution of discrepant results was 92.2 % and 99.5 %, respectively. Lowest specificity of the FTD assay was observed for human bocavirus. Multiple detections were recorded in 33/369 (8.9 %) of the samples by monoplex PCR and in 43/369 (11.7 %) using the FTD assay. The most prevalent viruses were respiratory syncytial virus and human metapneumovirus. Only pandemic influenza virus A/H1N1 (2009), and not seasonal influenza virus, was detected. Viruses other than influenza virus accounted for the majority of acute respiratory infections. The FTD assay can be easily implemented in general diagnostic laboratories and facilitate the optimization of patient-management schemes.