Label-free biosensing with functionalized nanopipette probes
Label-free biosensing with functionalized nanopipette probes
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DOI：
10.1073/pnas.0900306106
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发表时间：
2009-03-24
影响因子：
11.1
通讯作者：
Pourmand, Nader
中科院分区：
文献类型：
--
作者：
Umehara, Senkei;Karhanek, Miloslav;Pourmand, Nader
纳米移液器技术可以根据大小、形状和电荷的差异独特地识别蛋白质等生物分子。这些差异是通过检测蛋白质与涂有探针分子的纳米移液器尖端相互作用时离子电流的变化来确定的。在这里，我们展示了纳米移液器尖端表面上的静电、生物素-链霉亲和素和抗体-抗原相互作用影响流过 50 nm 孔的离子电流。高电荷聚合物与玻璃表面相互作用调节纳米移液管电极的整流特性。附着在表面的探针与其靶蛋白之间基于亲和力的结合会由于部分阻断或表面电荷的改变而引起离子电流的变化。这些发现表明，用适当的分子识别元件功能化的纳米移液器可以用作生物医学和生物研究中的纳米传感器。
Nanopipette technology can uniquely identify biomolecules such as proteins based on differences in size, shape, and electrical charge. These differences are determined by the detection of changes in ionic current as the proteins interact with the nanopipette tip coated with probe molecules. Here we show that electrostatic, biotin-streptavidin, and antibody-antigen interactions on the nanopipette tip surface affect ionic current flowing through a 50-nm pore. Highly charged polymers interacting with the glass surface modulated the rectification property of the nanopipette electrode. Affinity-based binding between the probes tethered to the surface and their target proteins caused a change in the ionic current due to a partial blockade or an altered surface charge. These findings suggest that nanopipettes functionalized with appropriate molecular recognition elements can be used as nanosensors in biomedical and biological research.