Caenorhabditis elegans vulval cell fate patterning
Caenorhabditis elegans vulval cell fate patterning
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DOI：
10.1088/1478-3975/9/4/045001
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发表时间：
2012-08-01
期刊：
影响因子：
2
通讯作者：
Felix, Marie-Anne
中科院分区：
文献类型：
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作者：
Felix, Marie-Anne
在一排感受态细胞中的三个细胞命运的空间模式的例子是线虫秀丽隐杆线虫的外阴发育。构成命运指定基础的细胞间信号网络已得到充分理解，但定量方面仍有待阐明。网络的定量模型使我们能够测试参数变化对细胞命运模式输出的影响。在使我们能够达到野生型模式的参数集中，可以发现三种命运的两种一般发育模式化机制：顺序诱导和基于形态发生素的诱导，前者对参数变化更鲁棒。在实验上，外阴细胞命运模式对随机和环境挑战是稳健的，并且可以检测到微小的变异。唯一的例外是前细胞P3.p的命运，它对随机变异和自发突变敏感，也是进化最快的。其他外阴前体细胞的命运可能会受到突变的影响，但几乎没有自然变异，这表明稳定选择。尽管这种命运模式的保护，不同的小杆线虫物种不同的系统扰动的反应。在定量模型中，不同的参数集可以重建它们对扰动的响应，这表明小杆线虫物种之间的网络变化可能是定量的。网络重新布线可能发生在更长的进化尺度上。
The spatial patterning of three cell fates in a row of competent cells is exemplified by vulva development in the nematode Caenorhabditis elegans. The intercellular signaling network that underlies fate specification is well understood, yet quantitative aspects remain to be elucidated. Quantitative models of the network allow us to test the effect of parameter variation on the cell fate pattern output. Among the parameter sets that allow us to reach the wild-type pattern, two general developmental patterning mechanisms of the three fates can be found: sequential inductions and morphogen-based induction, the former being more robust to parameter variation. Experimentally, the vulval cell fate pattern is robust to stochastic and environmental challenges, and minor variants can be detected. The exception is the fate of the anterior cell, P3.p, which is sensitive to stochastic variation and spontaneous mutation, and is also evolving the fastest. Other vulval precursor cell fates can be affected by mutation, yet little natural variation can be found, suggesting stabilizing selection. Despite this fate pattern conservation, different Caenorhabditis species respond differently to perturbations of the system. In the quantitative models, different parameter sets can reconstitute their response to perturbation, suggesting that network variation among Caenorhabditis species may be quantitative. Network rewiring likely occurred at longer evolutionary scales.