Comparative transcriptome RNA-Seq analysis of Listeria monocytogenes with sodium lactate adaptation
Comparative transcriptome RNA-Seq analysis of Listeria monocytogenes with sodium lactate adaptation
复制标题
单核细胞增生李斯特氏菌与乳酸钠适应的比较转录组 RNA-Seq 分析
DOI：
10.1016/j.foodcont.2018.03.044
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发表时间：
2018-09
期刊：
影响因子：
6
通讯作者：
Liu Yanhong
中科院分区：
文献类型：
--
作者：
Suo Yujuan;Gao Shigang;Baranzoni Gian Marco;Xie Yanping;Liu Yanhong
单核细胞增生性李斯特菌是一种重要的食源性致病菌，可引起高死亡率的李斯特菌病。乳酸钠已被用作肉制品中的抗菌剂，以抑制食源性病原体的生长。然而，单核细胞增生李斯特菌对乳酸钠的适应机制尚不清楚。本文研究了4%乳酸钠对即食肉中单核细胞增生李斯特菌的生长和存活的影响。进行RNA-Seq以比较在含有和不含4%乳酸钠的脑心浸液肉汤中培养的细胞之间的转录谱，以鉴定差异表达基因（DEG）。从响应于乳酸钠处理的RNA-Seq分析中鉴定了DEG（766），包括329个上调基因和437个下调基因。使用定量逆转录PCR（qRT-PCR）测定验证RNA-Seq数据。DEG包括参与信号转导、ABC转运蛋白和PTS系统等途径的基因。有趣的是，参与细菌趋化性和鞭毛组装的DEG被完全下调，表明鞭毛合成被乳酸钠处理抑制。最重要的是，诱导毒力相关基因，表明乳酸钠可能增加单核细胞增生李斯特菌的毒力潜力。我们的研究提供了对乳酸钠处理的单核细胞增生李斯特菌适应机制的见解，并可能有助于寻找与乳酸钠联合使用对RTE肉中单核细胞增生李斯特菌具有协同作用的策略。
Listeria monocytogenes is an important foodborne pathogen that can cause listeriosis with high mortality rates. Sodium lactate has been used as an antimicrobial in meat products to inhibit the growth of foodborne pathogens. However, the adaptation mechanism ofL. monocytogenesto sodium lactate remains unclear. In this paper, the growth/survival ofL. monocytogeneswas determined in ready-to-eat (RTE) meats with 4% sodium lactate treatment. RNA-Seq was conducted to compare the transcriptional profiles between cells cultured in Brain Heart Infusion broth with and without 4% sodium lactate to identify differentially expressed genes (DEGs). DEGs (766) including 329 up-regulated and 437 down-regulated genes were identified from RNA-Seq analysis in response to sodium lactate treatment. RNA-Seq data were validated using quantitative reverse transcription PCR (qRT-PCR) assays. The DEGs include genes involved in pathways including signal transduction, ABC transporter and PTS systems. Interestingly, the DEGs involved in bacterial chemotaxis and flagellar assembly were exclusively down-regulated, indicating that flagellar synthesis was inhibited by sodium lactate treatment. Most importantly, virulence-related genes were induced, suggesting that sodium lactate may increase the virulence potential ofL. monocytogenes. Our study provides insight on the adaptation mechanism ofL. monocytogeneswith treatment of sodium lactate, and may aid in searching for strategies that may have a synergistic effect in combination with sodium lactate to inhibitL. monocytogenesin RTE meat.
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影响因子：
4.4
作者：
C. D. Carroll;C. Alvarado;M. Brashears;L. Thompson;J. Boyce
通讯作者：
C. D. Carroll;C. Alvarado;M. Brashears;L. Thompson;J. Boyce
影响因子：
14.9
作者：
Ritchie ME;Phipson B;Wu D;Hu Y;Law CW;Shi W;Smyth GK
通讯作者：
Smyth GK
DOI：
10.1002/anie.201602974
发表时间：
2016-07-25
期刊：
Angewandte Chemie (International ed. in English)
影响因子：
--
作者：
Tal-Gan Y;Ivancic M;Cornilescu G;Yang T;Blackwell HE
通讯作者：
Blackwell HE
影响因子：
4.9
作者：
H. Snyder;Stephanie L. Kellogg;Laura M Skarda;Jaime L. Little;C. Kristich
通讯作者：
H. Snyder;Stephanie L. Kellogg;Laura M Skarda;Jaime L. Little;C. Kristich
影响因子：
3.2
作者：
Morimoto, Yusuke V.;Nakamura, Shuichi;Minamino, Tohru
通讯作者：
Minamino, Tohru