NITRIC-OXIDE SYNTHASE ACTIVITY IS ELEVATED IN BRAIN MICROVESSELS IN ALZHEIMERS-DISEASE
NITRIC-OXIDE SYNTHASE ACTIVITY IS ELEVATED IN BRAIN MICROVESSELS IN ALZHEIMERS-DISEASE
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DOI：
10.1006/bbrc.1994.2716
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发表时间：
1994-11-30
影响因子：
3.1
通讯作者：
GRAMMAS, P
中科院分区：
文献类型：
--
作者：
DORHEIM, MA;TRACEY, WR;GRAMMAS, P
脑微循环在阿尔茨海默病中经历特定的生化变化。在这项研究中，我们比较了一氧化氮合酶（NOS）活性的脑微血管分离阿尔茨海默病和对照组的大脑。L-[H-3]-瓜氨酸是由L-[H-3]-精氨酸与一氧化氮（NO）生成的稳定副产物，作为NOS活性的指标进行测定。结果表明，从阿尔茨海默氏症脑中分离的微血管中的NOS活性显着增加。此外，使用内皮和诱导型NOS亚型的抗体，我们证明了阿尔茨海默氏症衍生血管中酶水平的显着增加。血管产生的NO（CNS中潜在的神经毒性介质）升高可能导致阿尔茨海默病中神经元对损伤和细胞死亡的易感性。(C)1994年出版社出版。
The cerebral microcirculation undergoes specific biochemical changes in Alzheimer's disease. In this study, we have compared the nitric oxide synthase (NOS) activity of brain microvessels isolated from Alzheimer and control brains. L-[H-3]-citrulline, the stable co-product generated with nitric oxide (NO) from L-[H-3]-arginine, was measured as an indicator of NOS activity. The results indicated a significant increase in NOS activity in microvessels isolated from Alzheimer brains. In addition, using antibodies to both the endothelial and inducible NOS isoforms, we demonstrated a significant increase in enzyme level in Alzheimer-derived vessels. Elevated vascular production of NO, a potentially neurotoxic mediator in the CNS, may contribute to the susceptibility of neurons to injury and cell death in Alzheimer's disease. (C) 1994 Academic Press, Inc.