Intragraft IL-15 transcripts are increased in human renal allograft rejection
Intragraft IL-15 transcripts are increased in human renal allograft rejection
复制标题
DOI：
10.1097/00007890-199608270-00020
复制
发表时间：
1996-08-27
期刊：
影响因子：
6.2
通讯作者：
Strom, TB
中科院分区：
文献类型：
--
作者：
Pavlakis, M;Strehlau, J;Strom, TB
IL-15是一种由多种细胞产生的新型生长因子，以类似于IL-2的方式刺激T细胞增殖。在临床上明显的排斥反应时，在排斥的人肾移植物中通常不发现IL-2转录物。然而，T细胞增殖随着排斥的进展而继续。我们推测，IL-15可能是积极转录在临床排斥反应和帐户，至少部分，为正在进行的T细胞扩增。从肾活检组织中提取RNA，反转录成cDNA，作为竞争性PCR的模板。在45例活检标本中仅3例检测到IL-2 mRNA。在所有肾活检标本中均检测到IL-15转录本，与非排斥性肾移植物相比，在排斥性肾移植物中获得的标本中IL-15转录本显著增加。IL-15的转录与排斥反应有关，可能在T细胞介导的排斥反应中起重要作用。
IL-15, a novel growth factor made by a variety of cells, stimulates T cell proliferation in a fashion similar to IL-2. IL-2 transcripts are not routinely found in rejecting human renal allografts at the time of clinically evident rejection. However, T cell proliferation continues as the rejection progresses. We postulated that IL-15 may be actively transcribed during clinical rejection and account, at least in part, for the ongoing T cell expansion. RNA was extracted from renal biopsies and reverse transcribed to cDNA which was used as template for competitive PCR. IL-2 mRNA was detected in just 3 of the 45 biopsy samples. IL-15 transcripts were detected in all renal biopsy specimens and was significantly increased in specimens obtained from rejecting as compared with nonrejecting renal allografts. IL-15 transcription correlates with rejection and may play an important role in T cell mediated rejection.