Isolation of enteric pathogens from patients with travelers' diarrhea using fecal transport media
Isolation of enteric pathogens from patients with travelers' diarrhea using fecal transport media
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使用粪便运输介质分离旅行者腹泻患者的肠道病原体
DOI：
10.1111/j.1574-6968.1984.tb01036.x
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发表时间：
1984
影响因子：
2.1
通讯作者：
H. Dupont
中科院分区：
文献类型：
--
作者：
D. Morgan;Philip C. Johnson;A. West;L. Wood;C. Ericsson;H. Dupont
我们研究了两瓶粪便传输系统：甘油缓冲盐水(GBS)和抗生素改良Cary-Blair(CBA)将粪便样本传输到肠道疾病参考实验室的有效性。在一项盲法研究中，我们比较了在野外实验室迅速培养41个标本的结果与在参考实验室继代培养GBS和CBA的结果。从每个实验室72%的病例中分离出一种病原体。此外，从接种于GBS和CBA的45个样品中的32个样品中分离到一株病原菌，并将其运送到参考实验室进行传代培养。这些结果表明，在研究世界偏远地区腹泻的病原学时，GBS和CBA是将标本运送到参考实验室的有效手段。
We investigated the effectiveness of a two-vial fecal transport system: glycerol-buffered saline (GBS) and modified Cary-Blair with antibiotics (CBA) to transport stool samples to an enteric disease reference laboratory. In a blind study we compared the results of culturing 41 specimens promptly in the field laboratory to subculturing GBS and CBA in the reference laboratory. A pathogen was isolated from 72% of the cases in each laboratory. In addition, a pathogen was isolated from 32 of 45 samples inoculated into GBS and CBA and transported to the reference laboratory for subculture. These results demonstrate that GBS and CBA are effective means of transporting specimens to a reference laboratory when studying the etiology of diarrhea in remote parts of the world.