Red American ginseng:: Ginsenoside constituents and antiproliferative activities of heat-processed Panax quinquefolius roots
Red American ginseng:: Ginsenoside constituents and antiproliferative activities of heat-processed Panax quinquefolius roots
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DOI：
10.1055/s-2007-981524
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发表时间：
2007-06-01
期刊：
影响因子：
2.7
通讯作者：
Yuan, Chun-Su
中科院分区：
文献类型：
--
作者：
Wang, Chong-Zhi;Aung, Han H.;Yuan, Chun-Su
红亚洲人参（Panax ginseng C. A. Meyer，五加科）在许多东方国家使用。本文对清蒸西洋参皂苷成分及其抗癌活性进行了研究。根进行了评估。采用高效液相色谱法测定了12种黄芪皂苷的含量。经100-120 ℃蒸煮1h和120 ℃蒸煮0.5 ~ 4 h后，7种皂甙含量下降，5种皂甙含量增加。在120 ℃下蒸0.5-3 h，以前公认的抗癌化合物--通过改良的三色染色（MTS）方法，使用三种癌细胞系（SW-480、HT-29、NSCLC）来测定未蒸煮和蒸煮（120 ℃，1小时和2小时）西洋参根提取物的抗增殖作用。热处理显著提高了西洋参的抗增殖作用，且蒸制2 h的西洋参根提取物的抗增殖活性大于蒸制1 h的西洋参根提取物。本文还对白色和红色亚洲人参的化学成分和抗增殖活性进行了评价。对5种具有代表性的皂苷Rb 1、Rd、Re、Rg 2和Rg 3进行了研究。人参皂苷Rg 3的作用最强。西洋参的抗增殖活性随着人参皂苷Rg 3的增加而增强。
Red Asian ginseng (Panax ginseng C. A. Meyer, Araliaceae) is used in many Oriental countries. In this study, the saponin constituents and anticancer activities of steamed American ginseng (Panax quinquefolius L.) roots were evaluated. The contents of 12 ginsenosides in the roots were determined using high performance liquid chromatography (HPLC). After the steaming treatment (100-120 degrees C for 1 h and 120 degrees C for 0.5-4 h), the quantity of 7 ginsenosides decreased and that of 5 others increased. The content of ginsenoside Rg3, a previously recognized anticancer compound, increased significantly when the root was steamed at 120 degrees C for 0.5-3 h. The antiproliferative effects of unsteamed and steamed (120 degrees C for 1 h and 2 h) American ginseng root extracts were assayed by the modified trichrome stain (MTS) method using three cancer cell lines (SW-480, HT-29, NSCLC). Heat-processing increased the antiproliferative effect of American ginseng significantly, and the activity of the extract from roots steamed for 2 h was greater than that of roots steamed for 1 h. Chemical constituents and anti proliferative activities of white and red Asian ginseng have also been evaluated. Five representative ginsenosides, Rb1, Rd, Re, Rg2 and Rg3, were studied. Ginsenoside Rg3 had the most potent effect. The antiproliferative activities of red American ginseng are augmented when ginsenoside Rg3 is increased.