Infection studies with two highly pathogenic avian influenza strains (Vietnamese and Indonesian) in Pekin ducks (Anas platyrhynchos), with particular reference to clinical disease, tissue tropism and viral shedding
Infection studies with two highly pathogenic avian influenza strains (Vietnamese and Indonesian) in Pekin ducks (Anas platyrhynchos), with particular reference to clinical disease, tissue tropism and viral shedding
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DOI：
10.1080/03079450903055371
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发表时间：
2009-01-01
期刊：
影响因子：
2.8
通讯作者：
Daniels, Peter W.
中科院分区：
文献类型：
--
作者：
Bingham, John;Green, Diane J.;Daniels, Peter W.
北京鸭通过粘膜途径（口腔、鼻腔、眼部）感染两种欧亚系H5 N1高致病性禽流感病毒株之一：A/番鸭/越南/453/2004和A/鸭/Indramayu/BBVW/109/2006（来自印度尼西亚）。在攻毒后第1、2、3、5和7天，或当发病率严重到足以人道处死时，人道处死鸭子。通过观察临床体征和泄殖腔温度记录发病率;通过组织病理学表征疾病;通过免疫组织化学和组织样本病毒滴定研究组织嗜性;通过病毒分离和滴定口腔和泄殖腔拭子确定病毒脱落模式。越南株引起严重的发病率与发烧和抑郁症;印度尼西亚株只引起短暂的发烧。这两种病毒对相似范围的组织类型有偏好，但越南毒株的组织抗原量和组织病毒滴度要高得多。越南菌株引起严重的心肌炎和骨骼肌炎;两种菌株均引起非化脓性脑炎和一系列严重程度不同的其他炎症反应。感染的上皮组织主要是气囊，但抗原含量不高。鼻甲、气管、支气管上皮仅发生罕见的病毒感染。病毒从口服和泄殖腔途径脱落;攻毒后24 h首次检测到病毒，并持续至攻毒后第5天。从感染越南毒株的鸭拭子中检出的病毒流行率较高，表明该毒株可能比印度尼西亚毒株更适合鸭。
Pekin ducks were infected by the mucosal route (oral, nasal, ocular) with one of two strains of Eurasian lineage H5N1 highly pathogenic avian influenza virus: A/Muscovy duck/Vietnam/453/2004 and A/duck/Indramayu/BBVW/109/2006 (from Indonesia). Ducks were killed humanely on days 1, 2, 3, 5 and 7 after challenge, or whenever morbidity was severe enough to justify euthanasia. Morbidity was recorded by observation of clinical signs and cloacal temperatures; the disease was characterized by histopathology; tissue tropism was studied by immunohistochemistry and virus titration on tissue samples; and viral shedding patterns were determined by virus isolation and titration of oral and cloacal swabs. The Vietnamese strain caused severe morbidity with fever and depression; the Indonesian strain caused only transient fever. Both viruses had a predilection for a similar range of tissue types, but the quantity of tissue antigen and tissue virus titres were considerably higher with the Vietnamese strain. The Vietnamese strain caused severe myocarditis and skeletal myositis; both strains caused non-suppurative encephalitis and a range of other inflammatory reactions of varying severity. The principal epithelial tissue infected was that of the air sacs, but antigen was not abundant. Epithelium of the turbinates, trachea and bronchi had only rare infection with virus. Virus was shed from both the oral and cloacal routes; it was first detected 24 h after challenge and persisted until day 5 after challenge. The higher prevalence of virus from swabs from ducks infected with the Vietnamese strain indicates that this strain may be more adapted to ducks than the Indonesia strain.